MFA1 Antibody

Code CSB-PA341496XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MFA1 Polyclonal antibody
Uniprot No.
Target Names
MFA1
Alternative Names
MFA1 antibody; YDR461W antibody; D8035.5 antibody; Mating hormone A-factor 1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MFA1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
The active factor is excreted into the culture medium by haploid cells of the A mating type and acts on cells of the opposite mating type (type alpha). It mediates the conjugation process between the two types by inhibiting the initiation of DNA synthesis in type alpha cells and synchronizing them with type A.
Gene References into Functions
  1. Data indicate the substrate specificity of kexin protein(Kex2)using peptides derived from alpha-mating factor. PMID: 27720750
  2. Amino acid residues critical for N-terminal processing, protein secretion, and mating were identified from a collection of a-factor mutants. PMID: 16963638
  3. The loss of viability observed in telomerase-deficient haploids and diploids is less severe when both MATa and MATalpha are co-expressed than when a single MAT allele is expressed. PMID: 18627461
Subcellular Location
Cell membrane; Lipid-anchor; Cytoplasmic side.
Database Links

KEGG: sce:YDR461W

STRING: 4932.YDR461W

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