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mhpC Antibody

Datasheet
Code CSB-PA305183XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) mhpC Polyclonal antibody
Uniprot No.
P77044
Target Names
mhpC
Alternative Names
mhpC antibody; b0349 antibody; JW03402-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase antibody; EC 3.7.1.14 antibody; 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase antibody; 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase antibody; 2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) mhpC protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
ELISA Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

mhpC Proteins

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Target Background

Function
Catalyzes the cleavage of the C5-C6 bond of 2-hydroxy-6-oxononadienedioate and 2-hydroxy-6-oxononatrienedioate, a dienol ring fission product of the bacterial meta-cleavage pathway for degradation of phenylpropionic acid. MhpC shows some selectivity for the carboxylate of the side chain.
Gene References into Functions
  1. A catalytic mechanism is proposed involving stabilisation of reactive intermediates and activation of a nucleophilic water molecule by Ser110 PMID: 15663941
  2. A new line-broadened signal was observed upon incubation of the (13)C-labeled substrate with an H114A MhpC mutant, which is able to accept the 6-phenyl-containing substrate, on a shorter time scale. PMID: 17029401
  3. Replacement of Arg-188 in MhpC with Gln and Lys led to 200- and 40-fold decreases, respectively, in k(cat). PMID: 17029402
Protein Families
AB hydrolase superfamily, MhpC family
Database Links
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