MINE1 Antibody

Code CSB-PA883677XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MINE1 Polyclonal antibody
Uniprot No.
Target Names
MINE1
Alternative Names
MINE1 antibody; ARC12 antibody; At1g69390 antibody; F10D13.22 antibody; F23O10.25 antibody; Cell division topological specificity factor homolog antibody; chloroplastic antibody; AtMinE1 antibody; Protein ACCUMULATION AND REPLICATION OF CHLOROPLASTS 12 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana MINE1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Acts as a topological specificity factor during plastid division and specify plastid constriction sites. May contribute to gravitropism in stems and hypocotyls. Stimulates MIND1 ATPase activity. In cooperation with MIND1, prevents FtsZ ring formation anywhere outside of the mid-plastids.
Gene References into Functions
  1. In the cotyledons, floral organs, and root columella, the plastid size in the atminE1 mutant was significantly larger than in the wild type, while the plastid number per cell in atminE1 appeared to be inversely smaller than that in the wild type. PMID: 19966487
  2. MinE1 stimulates MinD1-mediated ATP hydrolysis, but in contrast to prokaryotes, stimulation occurs in the absence of membrane lipids PMID: 16014621
  3. Data support the model that MinE1 plays a pivotal role in selection of the plastid division site. PMID: 17855384
Subcellular Location
Plastid, chloroplast. Note=Dynamic protein that is restricted to a single spot at one end of chloroplasts or as two spots in close proximity normally towards one end of the chloroplast. Seems attached to membranes.
Protein Families
MinE family
Tissue Specificity
Expressed in green tissues, especially at the shoot apex. Also present in leaves, stems, buds, and flowers, especially in sepals, siliques (tip and base), and anthers (mostly in pollen grains).
Database Links

KEGG: ath:AT1G69390

STRING: 3702.AT1G69390.1

UniGene: At.27512

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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