mlrA Antibody

Code CSB-PA329252XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) mlrA Polyclonal antibody
Uniprot No.
Target Names
mlrA
Alternative Names
mlrA antibody; yehV antibody; b2127 antibody; JW2115HTH-type transcriptional regulator MlrA antibody; MerR-like regulator A antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) mlrA protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Activates transcription of csgD, the master regulator of biofilm formation, by binding to its promoter region. Also controls the transcription of cadC and ibaG. Part of a signaling cascade that regulates curli biosynthesis. The cascade is composed of two c-di-GMP control modules, in which c-di-GMP controlled by the DgcE/PdeH pair (module I) regulates the activity of the DgcM/PdeR pair (module II), which in turn regulates activity of the transcription factor MlrA.
Gene References into Functions
  1. identified MlrA as a positive factor of the csgD promoter by directly binding to its upstream region (-113 to -146) with a palindromic sequence of AAAATTGTACA(12N)TGTACAATTTT between the binding sites of two activators, IHF and OmpR PMID: 20874755
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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