Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) mod(mdg4) Polyclonal antibody

Uniprot No.

Q86B87

Target Names

mod(mdg4)

Alternative Names

mod(mdg4) antibody; bpd antibody; doom antibody; E(var)3-93D antibody; CG32491Modifier of mdg4 antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) mod(mdg4) protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Component of the gypsy chromatin insulator complex which is required for the function of the gypsy chromatin insulator and other endogenous chromatin insulators. Chromatin insulators are regulatory elements which establish independent domains of transcriptional activity within eukaryotic genomes. Insulators have two defining properties; they can block the communication between an enhancer and a promoter when placed between them and can also buffer transgenes from position effect variegation (PEV). Insulators are proposed to structure the chromatin fiber into independent domains of differing transcriptional potential by promoting the formation of distinct chromatin loops. This chromatin looping may involve the formation of insulator bodies, where homotypic interactions between individual subunits of the insulator complex could promote the clustering of widely spaced insulators at the nuclear periphery. Within the gypsy insulator complex, this protein may control the nature of the repressive effect of su(Hw): in the absence of mod(mdg4) protein, su(Hw) exerts a bidirectional silencing effect, whereas in the presence of mod(mdg4), the silencing effect is unidirectional. Isoform H is specifically required to maintain the pairing of achiasmate homologs in male meiosis I which is mediated by the rDNA repeats on the achiasmate X-Y bivalents. Isoform H also plays a role in apoptotic regulatory pathways.

Gene References into Functions

Mod(mdg4) directly interacts with mtACP1A and mtACP1B proteins. PMID: 27025476
Results show that mod(mdg4) pre-mRNAs are alternatively spliced, and a very significant fraction of the mature mod(mdg4) mRNAs are formed by trans-splicing. PMID: 24526065
The phenotypes that emerge in the context of wild-type levels of the HP1 and Mod(mdg4) proteins might be part of an adaptive response to the environment. PMID: 23636736
Trans-splicing takes place between transcripts produced on two different chromosomes to produce mod(mdg4) 2.2 protein PMID: 11973303
functional correlation between the large number of different isoforms and the pleiotropic mutant phenotypes of most mod(mdg4) mutations. Putative function of Mod(mdg4) proteins as chromatin modulators involved in higher order chromatin domains PMID: 12723696
the absence of the Mod(mdg4) protein allows the Su(Hw) insulator to function differently PMID: 12856417
Trans-splicing of the mod(mdg4) complex locus is conserved between the distantly related species Drosophila melanogaster and D. virilis. PMID: 15520256
analysis of MDG4 (Gypsy) sequences in the Drosophila melanogaster genome PMID: 15846993
These data suggest that the Mod(mdg4)-67.2 BTB domain confers novel activities to gypsy insulator function. PMID: 17101769
A mutation in the common region of mod(mdg4), Z3-3401, that causes qualitatively similar phenotypes as the MNM-specific alleles but disrupts X-Y segregation much more drastically than autosomal segregation, is described. PMID: 17277376
Mod(mdg4)devoid of the Q-rich domain supports the function of Su(Hw)-dependent insulators and efficiently binds to correct insulators on the chromosome, but does not form or enter the Su(*Hw)-marked nuclear speckles. PMID: 18369369

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Subcellular Location

Nucleus. Chromosome.

Database Links

KEGG: dme:Dmel_CG32491

STRING: 7227.FBpp0292464

UniGene: Dm.20407

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mod(mdg4) Antibody

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