MMS22 Antibody

Code CSB-PA594469XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MMS22 Polyclonal antibody
Uniprot No.
Target Names
MMS22
Alternative Names
MMS22 antibody; SLM2 antibody; YLR320WE3 ubiquitin-protein ligase substrate receptor MMS22 antibody; Methyl methanesulfonate-sensitivity protein 22 antibody; Synthetically lethal with MCM10 protein 2 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MMS22 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Substrate targeting component of a cullin-RING-based E3 ubiquitin-protein ligase complex RTT101(MMS1-MMS22). RTT101(MMS1-MMS22) promotes fork progression through damaged DNA or natural pause sites by stabilizing replication proteins like the replication fork-pausing complex (FPC) and leading-strand polymerase at stalled replication forks. RTT101(MMS1-MMS22) ubiquitinates the acetylated histones H3K56ac-H4 at lysine residues H3K121, H3K122 and H3K125. Ubiquitination is required for efficient histone deposition during replication-coupled nucleosome assembly, probably by facilitating the transfer of H3-H4 from ASF1 to other chaperones involved in histone deposition.
Gene References into Functions
  1. The DNA repair protein Mms22 is required for double strand break repair, and recruited to chromatin in a ubiquitin-dependent manner as a result of DNA damage. PMID: 20174551
  2. Mms22p protects cells from topoisomerase II-mediated DNA damage. PMID: 15718301
  3. cells lacking MMS22 have severly defective recombination between sister chromatids or between homologous chromosomes induced by agents that block replisomes and are defective in recovery from Methylmethane Sulfonate-induced replisome stalling PMID: 18321796
Subcellular Location
Nucleus. Note=Nuclear punctate foci structures.
Protein Families
MMS22 family
Database Links

KEGG: sce:YLR320W

STRING: 4932.YLR320W

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