mnmC Antibody

Code CSB-PA304368XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) mnmC Polyclonal antibody
Uniprot No.
Target Names
mnmC
Alternative Names
mnmC antibody; yfcK antibody; b2324 antibody; JW5380tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC antibody; tRNA mnm(5)s(2)U biosynthesis bifunctional protein) [Includes: tRNA antibody; mnm(5)s(2)U34)-methyltransferase antibody; EC 2.1.1.61); FAD-dependent cmnm(5)s(2)U34 oxidoreductase antibody; EC 1.5.-.-)] antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) mnmC protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the last two steps in the biosynthesis of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl group from S-adenosyl-L-methionine to nm(5)s(2)U34, to form mnm(5)s(2)U34.
Gene References into Functions
  1. Investigation of the activities and the tRNA specificity of the two MnmC domains. PMID: 24293650
  2. The structures of MnmC also reveal the detailed atomic interactions at the interdomain interface and provide spatial restraints relevant to the overall catalytic mechanism. PMID: 23617613
Subcellular Location
Cytoplasm.
Protein Families
Methyltransferase superfamily, tRNA (mnm(5)s(2)U34)-methyltransferase family; DAO family
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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