Uba4 Antibody

Code CSB-PA894708XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Uba4 Polyclonal antibody
Uniprot No.
Target Names
Uba4
Alternative Names
Uba4 antibody; CG13090 antibody; Adenylyltransferase and sulfurtransferase MOCS3 antibody; Molybdenum cofactor synthesis protein 3 antibody; Ubiquitin activating enzyme 4) [Includes: Molybdopterin-synthase adenylyltransferase antibody; EC 2.7.7.80 antibody; Adenylyltransferase MOCS3 antibody; Sulfur carrier protein MOCS2A adenylyltransferase); Molybdopterin-synthase sulfurtransferase antibody; EC 2.8.1.11 antibody; Sulfur carrier protein MOCS2A sulfurtransferase antibody; Sulfurtransferase MOCS3)] antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Uba4 protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.
Subcellular Location
Cytoplasm.
Protein Families
HesA/MoeB/ThiF family, UBA4 subfamily
Database Links

KEGG: dme:Dmel_CG13090

STRING: 7227.FBpp0079295

UniGene: Dm.5113

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