fml1 Antibody

Code CSB-PA892026XA01SXV
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Product Details

Full Product Name
Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) fml1 Polyclonal antibody
Uniprot No.
Target Names
fml1
Alternative Names
fml1 antibody; SPAC9.05 antibody; ATP-dependent DNA helicase fml1 antibody; EC 3.6.4.12 antibody; FANCM-like protein 1 antibody
Raised in
Rabbit
Species Reactivity
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Immunogen
Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) fml1 protein
Immunogen Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
ATP-dependent DNA helicase involved in DNA damage repair by homologous recombination and in genome maintenance. Capable of unwinding D-loops. Plays a role in limiting crossover recombinantion during mitotic DNA double-strand break (DSB) repair. Component of a FANCM-MHF complex which promotes gene conversion at blocked replication forks, probably by reversal of the stalled fork. FANCM-MHF also promotes non-crossover recombination in meiotic cells.
Gene References into Functions
  1. Mutations that impair ATP hydrolysis of Fml1 block DNA unwinding without blocking DNA binding. PMID: 22844101
  2. findings show that Fml1, the Fanconi anemia complementation group M (FANCM)-ortholog of Schizosaccharomyces pombe, directs the formation of non-crossovers (NCOs)during meiosis in competition with the Mus81-dependent pro-crossover (CO) pathway PMID: 22723423
Subcellular Location
Cytoplasm. Nucleus, nucleolus.
Protein Families
DEAD box helicase family, DEAH subfamily, FANCM sub-subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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