Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans mpk-1 Polyclonal antibody

Uniprot No.

P39745

Target Names

mpk-1

Alternative Names

mpk-1 antibody; sur-1 antibody; F43C1.2 antibody; Mitogen-activated protein kinase mpk-1 antibody; EC 2.7.11.24 antibody; MAP kinase sur-1 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans mpk-1 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Function in let-60 Ras signaling pathway; acts downstream of lin-45 raf kinase, but before the lin-1 gene product in controlling vulval cell differentiation. Plays a negative role in proximal germline proliferation in the mitotic zone. Required for progression of developing oocytes through the pachytene stage. In oocytes, inhibits the activity of the chloride channel clh-3, likely by activating gck-3. Plays a role in response to M.nematophilum-mediated bacterial infection by promoting tail swelling and preventing constipation. Involved in fluid homeostasis. In addition, involved in the up-regulation of lysozyme ilys-3 expression in the intestine in responses to M.nematophilum-mediated bacterial infection. By phosphorylating transcription factor skn-1 (isoform c) may play a role in increasing life span downstream of lin-45, let-60 and mek-2. By up-regulating cep-1 and down-regulating gld-1 expression in the late pachytene stage, plays a role in germline apoptosis in response to DNA damage. Regulates egl-1 expression in response to DNA damage, probably upstream of cep-1.; Suppresses germline tumor formation by preventing the dedifferentiation of secondary spermatocytes probably upstream of rskn-1.

Gene References into Functions

The results suggest that the MPK-1/ERK regulatory network, including FBF-1, FBF-2, and LIP-1, controls the number of sperm by regulating the timing of the sperm-oocyte switch in C. elegans. PMID: 28782521
data suggest that KIN-18 coordinates meiotic progression by modulating the timing of MPK-1 activation and the progression of recombination events PMID: 26510792
We show that MPK-1 signaling plays important developmental roles in pachytene progression and in regulating CEP-1 expression in pachytene, and a possible direct role in DNA damage induced apoptosis PMID: 21901106
In the proximal germline, sperm-dependent physiological MPK-1 activation results in phosphorylation-based inactivation of NOS-3, FEM-CUL-2-mediated degradation of TRA-1 and the promotion of membrane organization during oogenesis. PMID: 21571224
C. elegans ERK kinase MPK-1 or ERK phosphorylating/activating kinase MEK-2 constitutively activates native ClC anion channel CLH-3b. PMID: 21160027
Data indicates that MAP kinase signaling antagonizes PAR-1 signaling during early embryonic polarization. PMID: 19720857
GCK-1 is a negative regulator of MAP kinase activation and apoptosis in the C. elegans germline PMID: 19826475
MPK-1 thus may act not only in the control of individual processes but also in the coordination of contemporaneous processes and the integration of sequential processes in germline development PMID: 18073423

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Protein Families

Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily

Tissue Specificity

Expressed in cells lining the rectum. Isoform a is expressed in nervous system, body wall muscles and posterior intestine. Isoform b expression may be restricted to germline.

Database Links

KEGG: cel:CELE_F43C1.2

STRING: 6239.F43C1.2b

UniGene: Cel.34032

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mpk-1 Antibody

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