MPK12 Antibody

Datasheet

Code	CSB-PA013451XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MPK12 Polyclonal antibody

Uniprot No.

Q8GYQ5

Target Names

MPK12

Alternative Names

MPK12 antibody; At2g46070 antibody; T3F17.28 antibody; Mitogen-activated protein kinase 12 antibody; AtMPK12 antibody; MAP kinase 12 antibody; EC 2.7.11.24 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana MPK12 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Negative regulator of the auxin transduction signaling pathway. Involved in stomatal movement regulation by phosphorylating and repressing HT1 and HT1-mediated GHR1 phosphorylation. Required for CO(2)-mediated stomatal movements (e.g. closure).

Gene References into Functions

Study propose a model for sequential roles of MPK12, HT1, and GHR1 in the ABA-independent regulation of SLAC1 during CO2-induced stomatal closure. PMID: 27694184
MPK9 and MPK12 are positive regulators of salicylic acid signaling in Arabidopsis guard cells. PMID: 28387156
MPK9 and MPK12 are key regulators mediating both abscisic acid (ABA) and Methyl jasmonate (MeJA) signalling in guard cells. PMID: 25703019
MPK9 and MPK12 function redundantly downstream of extracellular reactive oxygen production and intracellular accumulation, cytosolic alkalisation and Ca(2+)cytosolic oscillation in yeast elcictor-induced stomatal closure PMID: 23043299
MPK9 and MPK12 act downstream of ROS and cytosolic Ca2+ and upstream of anion channels in the guard cell abscisic acid signaling cascade. PMID: 22067989
MAP kinases MPK9 and MPK12 are preferentially expressed in guard cells and positively regulate ROS-mediated ABA signaling. PMID: 19910530
MPK12 is both a physiological substrate of IBR5 and a novel negative regulator of auxin signaling. PMID: 19000167

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Subcellular Location

Nucleus. Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Protein Families

Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily

Tissue Specificity

Expressed in seedlings, roots, stems, leaves, flowers and siliques, mostly around stomata.

Database Links

KEGG: ath:AT2G46070

STRING: 3702.AT2G46070.1

UniGene: At.36555

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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MPK12 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background