Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MPK4 Polyclonal antibody

Uniprot No.

Q39024

Target Names

MPK4

Alternative Names

MPK4 antibody; At4g01370 antibody; F2N1.1 antibody; F2N1_2-t antibody; Mitogen-activated protein kinase 4 antibody; AtMPK4 antibody; MAP kinase 4 antibody; EC 2.7.11.24 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana MPK4 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

The ANPs-MKK6-MPK4 module is involved in the regulation of plant cytokinesis during meiosis and mitosis. Essential to promote the progression of cytokinesis and for cellularization (formation of the cell plate) during male-specific meiosis. Involved in cortical microtubules organization and stabilization by regulating the phosphorylation state of microtubule-associated proteins such as MAP65-1. Involved in root hair development process. Negative regulator of systemic acquired resistance (SAR) and salicylic acid- (SA) mediated defense response. Required for jasmonic acid- (JA) mediated defense gene expression. May regulate activity of transcription factor controlling pathogenesis-related (PR) gene expression. Seems to act independently of the SAR regulatory protein NPR1 (Nonexpresser of PR1). Phosphorylates MKS1 and transcription factors WRKY25 and WRKY33. The MEKK1, MKK1/MKK2 and MPK4 function in a signaling pathway that modulates the expression of genes responding to biotic and abiotic stresses and also plays an important role in pathogen defense by negatively regulating innate immunity. Phosphorylates MEKK2 upon treatment with flg22. Involved in stomatal movement regulation by repressing HT1 and HT1-mediated GHR1 phosphorylation.

Gene References into Functions

H2S inhibited the opening of stomata under cold stress, which required the participation of MPK4. In conclusion, MPK4 is a downstream component of H2S-related cold-stress resistance, and H2S and MPK4 both regulated the cold response genes and stomatal movement to response the cold stress. PMID: 29024849
AvrRpt2 specifically blocks the flagellin-induced activation of MPK4. PMID: 27208280
Data show that MPK4 phosphorylation of MYB75 increases its stability and is essential for light-induced anthocyanin accumulation. Our findings reveal an important role for a MAPK pathway in light signal transduction. PMID: 27811015
ASR3 functions as a transcriptional repressor regulated by microbe-associated molecular patterns-activated MPK4 to fine-tune plant immune gene expression. PMID: 25770109
Mitogen-activated protein kinase 4 is a salicylic acid-independent regulator of growth but not of photosynthesis in Arabidopsis PMID: 24874867
Arabidopsis MPK4 can interact with and be phosphorylated by the cytokinesis-related MAP kinase kinase, AtMKK6. PMID: 21575092
MPK4 activity was found to compromise effector-triggered immunity conditioned by the Toll Interleukin-1 Receptor-nucleotide binding (NB)-Leu-rich repeat (LRR) receptors RPS4 and RPP4 but not by the coiled coil-NB-LRR receptors RPM1 and RPS2. PMID: 23115249
MPK4 is shown to be involved in the regulation of mitosis/cytokinesis through modulation of the cell division plane and cytokinetic progression. PMID: 21155826
These results indicate that MPK4 is the downstream target of MKK6/ANQ in the regulation of cytokinesis in Arabidopsis and that MPK11 is also involved in cytokinesis. PMID: 21098735
Pseudomonas syringae effector protein AvrB interacts with Arabidopsis MPK4 and the HSP90 chaperone, and AvrB induces MPK4 activation in a manner promoted by HSP90 PMID: 20159621
Treatment with elevated levels of sodium chloride inhibits the growth of mpk4 transgenic plants; the mekk1;mpk4 double-mutant combination causes seedling lethality. PMID: 17572407
These data establish direct links between MPK4 and innate immunity and provide an example of how a plant MAP kinase can regulate gene expression by releasing transcription factors in the nucleus upon activation. PMID: 18650934

Hide All

Subcellular Location

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton.

Protein Families

Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily

Tissue Specificity

Ubiquitous. Expressed in the veins and stomatal guard cells of leaf plates, petioles, stem, roots and flowers.

Database Links

KEGG: ath:AT4G01370

STRING: 3702.AT4G01370.1

UniGene: At.19915

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MPK4 Antibody

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