MPK6 Antibody

Code CSB-PA013458XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MPK6 Polyclonal antibody
Uniprot No.
Target Names
MPK6
Alternative Names
MPK6 antibody; At2g43790 antibody; F18O19.10 antibody; Mitogen-activated protein kinase 6 antibody; AtMPK6 antibody; MAP kinase 6 antibody; EC 2.7.11.24 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana MPK6 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Mitogen-activated protein kinase (MAPK) which regulates abscisic acid (ABA) responses in a MAPKKK20-MKK5-MPK6 cascade involved in root growth (e.g. root cell division and elongation) and stomatal response. Involved in oxidative stress-mediated signaling cascade (such as ozone). Involved in the innate immune MAP kinase signaling cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6) downstream of bacterial flagellin receptor FLS2. May be involved in hypersensitive response (HR)-mediated signaling cascade by modulating LIP5 phosphorylation and subsequent multivesicular bodies (MVBs) trafficking. May phosphorylate regulators of WRKY transcription factors. Phosphorylates 1-aminocyclopropane-1-carboxylic acid synthases (ACS2 and ACS6) and may be involved in the regulation of bacterial elicitor flagellin-induced ethylene production. Regulates locally gene-mediated and basal resistance response to certain pathogens. May be involved in the cold and salinity stress-mediated MAP kinase signaling cascade (MEKK1, MKK1/MKK2 and MPK4/MPK6). MKK1-MPK6 module mediates abscisic acid (ABA)-dependent CAT1 expression with H(2)O(2) production and response to drought and salt stress. MKK1-MPK6 module is also involved in sugar signaling during the process of seed germination. MKK3-MPK6 module plays an important role in the jasmonate signal transduction pathway through the negative regulation of MYC2/JIN1 expression. MKK9-MPK3/MPK6 module phosphorylates and activates EIN3, leading to the promotion of EIN3-mediated transcription in ethylene signaling. MPK3/MPK6 cascade regulates camalexin synthesis through transcriptional regulation of the biosynthetic genes after pathogen infection. MKK9-MPK6 module positively regulates leaf senescence. YDA-MKK4/MKK5-MPK3/MPK6 module regulates stomatal cell fate before the guard mother cell (GMC) is specified. When activated, reinforces the feedback loop by phosphorylating BASL, and inhibits stomatal fate by phosphorylating SPCH. This MAPK cascade also functions downstream of the ER receptor in regulating coordinated local cell proliferation, which shapes the morphology of plant organs.
Gene References into Functions
  1. The Arabidopsis MKK4/MKK5-MPK6 cascade is an important player in the maternal control of embryogenesis. PMID: 29024034
  2. abscisic acid and jasmonate mediate inactivation of the immune-associated MAP kinases (MAPKs), MPK3 and MPK6, in Arabidopsis thaliana ABA induced expression of genes encoding the protein phosphatases 2C (PP2Cs), HAI1, HAI2, and HAI3 through ABF/AREB transcription factors PMID: 28652328
  3. Proteome changes associated with MPK4 and MPK6 deficiency in Arabidopsis roots. PMID: 27324189
  4. Results suggest that these mutants can be used to analyze the specific biological functions of MAP kinase 6 (MPK6). PMID: 27160427
  5. These data highlight MPK6 and MKP1 as components of an L-Glutamate pathway linking the auxin response, and cell division for primary root growth. PMID: 29344832
  6. Genetic and biochemical evidences not only show that MEK1-MPK6 cascade, AtRBOHD/F-dependent H2O2 and NIA1-dependent NO are all involved in dark-induced stomatal closure in Arabidopsis, also indicate that MEK1-MPK6 cascade functions via working downstream of H2O2 and upstream of NO. PMID: 28716416
  7. that MPK3/MPK6 phosphorylate and destabilize ICE1, which negatively regulates CBF expression and freezing tolerance in plants PMID: 29056553
  8. BASL polarization leads to elevated nuclear MPK6 signaling and lowered SPCH abundance in one of the two daughter cells. Therefore, two daughter cells are differentiated by BASL polarity-mediated differential suppression of SPCH, which may provide developmental plasticity in plant stem cell asymmetric cell division. PMID: 27746029
  9. results indicated that AIK1-MKK5-MPK6 functions in ABA responses and requires ABA-responsive gene expression to regulate ABA-inhibited root growth and cell division. The ABA signaling pathway regulates this MAPK cascade. PMID: 28494202
  10. MPK6 role in ultraviolet induced stomatal closure PMID: 27837091
  11. Study propose that the pathogen-responsive MPK3/MPK6 cascade and ABA are two essential signaling pathways that control, respectively, the organic acid metabolism and ion channels, two main branches of osmotic regulation in guard cells that function interdependently to control stomatal opening/closure. PMID: 28254778
  12. MPK6-mediated regulation of MYB15 plays an important role in cold stress signaling in Arabidopsis. PMID: 28510716
  13. Data report that MPK3/MPK6 and their substrate ERF6 promote the biosynthesis of IGSs and the conversion of I3G to 4MI3G, a target of PEN2/PEN3-dependent chemical defenses in plant immunity. PMID: 27081184
  14. Data show that the protein kinases MPK3 and MPK6 can both interact with SPOROCYTELESS/NOZZLE (SPL) in vitro and in vivo and can phosphorylate the SPL protein in vitro. PMID: 28209842
  15. AIK1-MKK5-MPK6 cascade functions in the abscisic acid regulation of primary root growth and stomatal response. PMID: 27913741
  16. A novel mechanism for PIN1 phosphorylation involving MKK7 and MPK6 in shoot branching regulation in Arabidopsis. PMID: 27618482
  17. MKK4, MKK5, MKK7, and MKK9, are responsible for the activation of MPK3 and MPK6 by melatonin, indicating that melatonin-mediated innate immunity is triggered by MAPK signaling through MKK4/5/7/9-MPK3/6 cascades. PMID: 26927635
  18. Mitogen-activated protein kinase 6 (MPK6) promoted C-terminal end of ORE3/EIN2 (CEND cleavage and nuclear translocation. Nuclear CEND accumulated ETHYLENE INSENSITIVE3 (EIN3), a transcription factor that accelerates MeJA-induced leaf senescence. PMID: 26507893
  19. Results demonstrated the contribution of MPK3 and MPK6 to riboflavin-induced resistance. PMID: 27054585
  20. MPK6 phosphorylates EB1c, but not EB1a, and has a role in maintaining regular planes of cell division under stress conditions. PMID: 26061286
  21. BES1 is identified as a unique direct substrate of MPK6 in Pathogen-associated molecular patterns (PAMP)-triggered immunity signaling. PMID: 25609555
  22. These results indicate that the MVB pathway is positively regulated by pathogen-responsive MPK3/6 through LIP5 phosphorylation and plays a critical role in plant immune system PMID: 25010425
  23. Ethylene (ET) affects auxin signaling through the central regulator CTR1 and that fungal-derived compounds, such as indole-3-acetic acid and indole-3-acetaldehyde, induce MPK6 activity. PMID: 26067203
  24. AGB1 interacts with AtMPK6 and may negatively regulate the abscissic acid signaling pathway. PMID: 25635681
  25. MPK6 controls hydrogen peroxide-induced root elongation by mediating Ca2+ influx across the plasma membrane of root cells in Arabidopsis seedlings. PMID: 25145265
  26. MPK6-mediated WRKY6 and Trx h5 transcriptional activation co-regulated the expression of the NPR1 gene and the monomerization of NPR1 protein, allowing it to enter the nucleus, thereby promoting salicylic acid-induced leaf senescence. PMID: 25210078
  27. MPK3 and MPK6 target a subclass of 'VQ-motif'-containing proteins to regulate immune responses. PMID: 24750137
  28. These results suggest that the MKK9-MPK3/MPK6 cascade is part of the phosphate signaling pathway in plants. PMID: 24865627
  29. YODA and MPK6 are involved in post-embryonic root development through an auxin-dependent mechanism regulating cell division and mitotic microtubule (PPB and phragmoplast) organization. PMID: 24923680
  30. retrograde signal transmission model is proposed starting with metabolite export through the triose phosphate/phosphate translocator with subsequent MPK6 activation leading to initiation of AP2/ERF-TF gene expression PMID: 24668746
  31. HSFA4A is a substrate of the MPK3/MPK6 signaling and that it regulates stress responses in Arabidopsis. PMID: 24676858
  32. establishment of an mpk3 mpk6 double mutant system using a chemical genetic approach provides a powerful tool to investigate the function of MPK3/MPK6 in the plant defense signaling pathway PMID: 24245741
  33. Loss of MPK3/MPK6 phosphorylation sites in WRKY34 compromises the function of WRKY34 in vivo. Epistasis interaction analysis confirmed that MPK6 belongs to the same genetic pathway of WRKY34 and WRKY2 PMID: 24830428
  34. MPK6 plays an important role during embryo development and acts as a repressor of primary and lateral root development. PMID: 24218326
  35. mpk6 decreased NaCl-induced the Na(+) accumulation by modifying plasma membrane (PM) Na(+) flux in root cells. There was a NaCl-induced increase of cytosolic Ca(2+) in mpk6 root cells, resulting from PM Ca(2+) influx. PMID: 24484955
  36. MPK3/MPK6 regulate multiple defense pathways in plant fungal resistance. PMID: 23524660
  37. These results suggest that MPK6 functions in both penetration and post-penetration resistance to M. oryzae in Arabidopsis thaliana. PMID: 23748771
  38. These data might provide new insight into the mechanisms that regulate reactive oxygen species-responsive gene transcription via a complex of MPK6, ERF6, and the ROSE7/GCC box under oxidative stress. PMID: 23300166
  39. these results suggest that phosphorylation by MPK6 is required for the functional role of ZAT6 in seed germination under salt and osmotic stress. PMID: 23257164
  40. The role of AtBI1 over-expression in delaying Methyl jasmonate-induced leaf senescence by suppressing the [Ca(2+)](cyt)-dependent activation of MPK6, thus providing a new insight into the function and mechanism of AtBI1 in plant senescence. PMID: 22563118
  41. These results suggest that ROS, but not abscisic acid, induces MPK6 activation as an upstream signal under drought conditions. PMID: 22975351
  42. A mpk6, but not a mpk3, null allele is able to suppress the effects of this dominant WAK2 mutation PMID: 22155845
  43. Data suggest that amino acids surrounding the phosphorylated serine residue of peptide substrates contribute to MPK6/MPK3 substrate specificity; MPK6/MPK3 activities appear to be important in growth of plant stomata. PMID: 22631074
  44. MAP Kinase 6-mediated activation of vacuolar processing enzyme modulates heat shock-induced programmed cell death in Arabidopsis PMID: 22497243
  45. These results indicate that the phosphorylation of MYB41 by MPK6 is required for the biological function of MYB41 in salt tolerance. PMID: 22575450
  46. The change in transcript profile of MAP2K9 and MAPK6 in leaves of A. thaliana ecotpe Columbia at early, middle and late stages of Alternaria blight infection, were determined. PMID: 21947882
  47. AtMPK6 activation was also triggered by phytosphingosine-1-phosphate (PHS-P) treatment independently of phytosphingosine/PHS-P balance. PMID: 22236066
  48. These results suggest that mRNA decapping through MPK6-DCP1-DCP5 pathway serves as a rapid response to dehydration stress in Arabidopsis. PMID: 22407295
  49. MKKK20 involved in the response to various abiotic stresses, especially osmotic stress, through its regulation of MPK6 activity PMID: 21969089
  50. MPK3 and MPK6 are negative regulators of the acute UV-B stress-resistance pathway, and are regulated by MKP1 in a UVR8-independent manner. PMID: 21790814

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Subcellular Location
Cytoplasm. Nucleus. Cytoplasm, cell cortex.
Protein Families
Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
Database Links

KEGG: ath:AT2G43790

STRING: 3702.AT2G43790.1

UniGene: At.22266

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