Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MPS3 Polyclonal antibody

Uniprot No.

P47069

Target Names

MPS3

Alternative Names

MPS3 antibody; NEP98 antibody; YJL019W antibody; J1310 antibody; J1315 antibody; YJL018W antibody; Spindle pole body assembly component MPS3 antibody; 98 kDa nuclear envelope protein antibody; Monopolar spindle protein 3 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MPS3 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Required for the first step of spindle pole body duplication in G1. Essential for nuclear division and fusion. Functions in sister chromatid cohesion establishment. Connects the spindle pole body with the nuclear envelope through its interaction with MPS2 and mediates meiotic bouquet formation and rapid chromosome movements in meiotic prophase. Functions as an integral membrane anchor for telomeres and is a nuclear receptor for the SIR4 pathway of telomere tethering and gene inactivation. recruits double-strand breaks (DSBs) to the nuclear periphery for chromosome healing.

Gene References into Functions

the response to a secretory defect required SUN protein Mps3, which was localized at the nuclear envelope and involved in spindle pole body assembly PMID: 27862269
We show that noncleavable Mps3 (Mps3-nc) inhibits centrosome separation during yeast meiosis. In addition, overexpression of mps3-nc in vegetative yeast cells also inhibits centrosome separation and is lethal PMID: 28609436
TFIIIC and Mps3 together direct the intranuclear positioning of a new class of S. cerevisiae genomic loci positioned at the nuclear periphery. PMID: 22496415
we propose that Mps3 facilitates insertion of SPBs(spindle pole body) in the nuclear membrane by modulating nuclear envelope composition. PMID: 22125491
These findings suggest that in meiosis, the Mps3 SUN domain is important for modulating chromosome motion events that act in meiotic chromosome juxtaposition and by extension, promoting proper morphogenesis of the synaptonemal complex. PMID: 22017544
These results suggest that the ribosome biogenesis factors Ebp2 and Rrs1 cooperate with Mps3 to mediate telomere clustering, but not telomere tethering, by binding Sir4. PMID: 21822217
H2A.Z is required to target a soluble Mps3 fragment to the nucleus and to localize full-length Mps3 in the inner nuclear membrane, indicating that H2A.Z has a novel chromatin-independent function in INM targeting of SUN proteins PMID: 21518795
Mps3p has a role in sister chromatid cohesion PMID: 15355977
The nuclear envelope, via Mps3p, plays a pivotal role in telomere foci formation. PMID: 17245108
Ndj1p and Mps3p are codependent for stable association with the telomeres PMID: 17495028
Bouquet formation and rapid prophase movements depend on NDJ1, MPS3, and a new member of this pathway, CSM4, which encodes a meiosis-specific nuclear envelope protein required specifically for telomere mobility. PMID: 18585352
identify the conserved Sad1-UNC-84 (SUN) domain protein Mps3 as the principal membrane anchor for this telomere anchoring pathway PMID: 19390087

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Subcellular Location

Nucleus membrane; Single-pass type II membrane protein. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Note=Localizes to the spindle pole body half bridge throughout the cell cycle.

Database Links

KEGG: sce:YJL019W

STRING: 4932.YJL019W

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