mreB Antibody

Code CSB-PA188034XA01BRJ
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Product Details

Full Product Name
Rabbit anti-Bacillus subtilis (strain 168) mreB Polyclonal antibody
Uniprot No.
Target Names
mreB
Alternative Names
mreB antibody; BSU28030 antibody; Cell shape-determining protein MreB antibody; Actin-like MreB protein antibody; Rod shape-determining protein MreB antibody
Raised in
Rabbit
Species Reactivity
Bacillus subtilis (strain 168)
Immunogen
Recombinant Bacillus subtilis (strain 168) mreB protein
Immunogen Species
Bacillus subtilis (strain 168)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Forms membrane-associated dynamic filaments that are essential for cell shape determination. Acts by regulating cell wall synthesis and cell elongation, and thus cell shape. A feedback loop between cell geometry and MreB localization may maintain elongated cell shape by targeting cell wall growth to regions of negative cell wall curvature. Filaments rotate around the cell circumference in concert with the cell wall synthesis enzymes. The process is driven by the cell wall synthesis machinery and does not depend on MreB polymerization. The active movement of the MreB cytoskeleton stimulates diffusion of membrane proteins. Organizes peptidoglycan synthesis in the lateral cell wall. Also required for proper chromosome segregation. Membrane fluidity influences MreB movement; in rich medium MreB movement is significantly decreased in a floA-floT mutant.
Gene References into Functions
  1. reveal another function for the MreB cytoskeleton and describe a mechanism by which viral DNA replication is organized at the bacterial membrane PMID: 19654094
  2. Data indicate that MreB forms membrane-associated filaments that coordinate bacterial cell wall synthesis and create specific membrane regions with increased fluidity (RIFs). PMID: 24603761
  3. study proposes a model in which soluble MreB organizes intracellular steps of peptidoglycan synthesis in the cytoplasm to feed the membrane-associated cell wall synthesizing machineries. PMID: 24261876
  4. WalR appears to coordinately and positively regulate transcription of sigI, mreBH and lytE under heat stress. PMID: 24125693
  5. Upon induction in Escherichia coli cells, B. subtilis MreB filaments push the cells into strikingly altered cell morphology. PMID: 21091501
  6. genetic and cytological links between MreB and the membrane, as well as with other actin like proteins; the connection of MreB with the chromosome PMID: 15745453
  7. These results suggest that the major role of MreB in B. subtilis lies in the control of cell diameter. PMID: 15752190
  8. These observations collectively suggest that additional factors interact with MreB to account for its complex dynamic behavior in cells. PMID: 19117023
  9. MreB filaments associate directly with the peptidoglycan biosynthetic machinery. Proper localization of PBP1 is dependent on MreB. PMID: 19192185
  10. mreB(B. licheniformis) but not mreB(Clostridium perfringens) can compensate for the loss of B. subtilis mreB. PMID: 19643765
  11. The lateral wall peptidoglycan synthesis is organized by the redundant action of MreB isoforms. PMID: 19659933

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Subcellular Location
Cytoplasm.
Protein Families
FtsA/MreB family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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