Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) Acp26Aa Polyclonal antibody

Uniprot No.

P10333

Target Names

Acp26Aa

Alternative Names

Acp26Aa antibody; Mst26Aa antibody; mst355a antibody; CG8982 antibody; Accessory gland-specific peptide 26Aa antibody; Male accessory gland secretory protein 355A antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) Acp26Aa protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Male seminal protein which enhances ovulation in female Drosophila by stimulating the release of oocytes by the ovary following mating. Acts by increasing octopamine (OA) neuronal signaling in the female genital tract leading to the postmating relaxation of the oviduct muscles. This activation of the OA signaling pathway is likely to indirectly contribute to the mating-dependent increase in the number of OA synaptic sites in the female reproductive tract.; Male seminal peptide which is able to enhance ovulation in female Drosophila.; Male seminal peptide which is able to enhance ovulation in female Drosophila.

Gene References into Functions

Data indicate that ovulin increases ovulation through octopamine (OA) neuronal signaling and suggest that seminal proteins can alter reproductive physiology by modulating known female pathways regulating reproduction. PMID: 24101486
Data show that the levels of a fecundity-stimulating Sfp (ovulin) were significantly lower in M females relative to V females, at the same time point shortly after the end of mating. PMID: 21628597
Results reveal a rather flexible sex peptide that lacks a compact folded structure in solution. PMID: 21439282
Because ovulin's self-interaction requires only small conserved domains, the rest of the molecule can be relatively tolerant to mutations. PMID: 20138215
ovulin's cleavage in mated females is not destructive and instead may liberate additional functional products with potential to modulate ovulation independently PMID: 15640356
Domains involved in ovulin's self-interaction form a conserved structural backbone for the protein, resulting in greater evolutionary flexibility at other sites. PMID: 17130459

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Subcellular Location

Secreted. Cytoplasm.

Tissue Specificity

Produced in the male accessory glands and secreted into seminal fluid (at protein level). Detected in the main cells and secondary cells of the accessory glands of 1 day old males (at protein level). In 5 day old males, confined to the secondary cells and

Database Links

KEGG: dme:Dmel_CG8982

STRING: 7227.FBpp0078786

UniGene: Dm.23389

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Acp26Aa Antibody

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