MSI1 Antibody

Code CSB-PA320262XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MSI1 Polyclonal antibody
Uniprot No.
Target Names
MSI1
Alternative Names
MSI1 antibody; CAC3 antibody; YBR195C antibody; YBR1405 antibody; Chromatin assembly factor 1 subunit p50 antibody; CAF-1 p50 subunit antibody; IRA1 multicopy suppressor antibody; Protein MSI1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MSI1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Acts as a component of chromatin assembly factor 1 (CAF-1), which assembles histone octamers onto replicating DNA in vitro. It performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. p50 and p60 form complexes with newly synthesized histones H3 and acetylated H4 in cell extracts. Independently, MSI1 is involved in regulation of the RAS/cAMP pathway via sequestration of NPR1.
Gene References into Functions
  1. Over-expression of YAK1 decreased the growth rate, but only in the presence of a functional MSI1 gene. PMID: 17321547
  2. Rho5p is a target of Msi1p/Npr1p regulation and the regulatory circuit involving phosphorylation and ubiquitination. PMID: 18621925
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
WD repeat RBAP46/RBAP48/MSI1 family
Database Links

KEGG: sce:YBR195C

STRING: 4932.YBR195C

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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