Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MSI4 Polyclonal antibody

Uniprot No.

O22607

Target Names

MSI4

Alternative Names

MSI4 antibody; ACG1 antibody; FVE antibody; At2g19520 antibody; F3P11.12 antibody; WD-40 repeat-containing protein MSI4 antibody; Altered cold-responsive gene 1 protein antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana MSI4 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of the flowering autonomous pathway which positively regulates flowering by promoting transcriptional repression of the flowering repressor FLC. May promote histone deacetylation at the FLC locus leading to the formation of repressive chromatin structures. Forms a histone deacetylase complex with HDA5, HDA6 and FLD that represses FLC gene expression to control flowering time. Also negatively regulates cold-responsive genes. Acts together with PDP1 and MSI5 to regulate the function of the PRC2 complex on FLC.

Gene References into Functions

We also demonstrate that PTM, a PHD transcription factor involved in chloroplast retrograde signaling, perceives such a signal and mediates transcriptional repression of FLC through recruitment of FVE, a component of the histone deacetylase complex. PMID: 27601637
Data propose these novel ATMSI4-associated proteins help form nucleoprotein complexes that determine pleiotropic functional properties of AtMSI4/FVE/ACG1 involving plant development PMID: 25600937
FVE is essential for the HOS1-mediated activation of FLC transcription. PMID: 24220632
Two phospho-beta-galactosidase, LacG1 and LacG2, are the primary enzymes for lactose utilization in Lb. gasseri. PMID: 22449746
we report that two Arabidopsis homologs of the human histone-binding proteins Retinoblastoma-Associated Protein 46/48 (RbAp46/48), known as MSI4 (or FVE) and MSI5, function in partial redundancy in chromatin silencing of various loci targeted by siRNAs PMID: 22102827
FVE, an Arabidopsis homologue of the retinoblastoma-associated protein that regulates flowering time and cold response, binds to chromatin as a large multiprotein complex. PMID: 21710206
These results suggest that there may be a conserved function between OsFVE and FVE in the control of flowering time. PMID: 18612241

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Subcellular Location

Nucleus.

Protein Families

WD repeat RBAP46/RBAP48/MSI1 family

Tissue Specificity

Expressed in rosette leaves, cauline leaves, main stems and developing fruits. Expressed at higher levels in roots and flowers.

Database Links

KEGG: ath:AT2G19520

STRING: 3702.AT2G19520.1

UniGene: At.20032

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MSI4 Antibody

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