msl-3 Antibody

Code CSB-PA342300XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) msl-3 Polyclonal antibody
Uniprot No.
Target Names
msl-3
Alternative Names
msl-3 antibody; CG8631 antibody; Protein male-specific lethal-3 antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) msl-3 protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
The MSL proteins are essential for elevating transcription of the single X chromosome in the male (X chromosome dosage compensation). Mle, msl-1 and msl-3 are colocalized on the X chromosome. Each of the MSL proteins requires all the other MSLs for wild-type X-chromosome binding.
Gene References into Functions
  1. MSL3 plays an important role in targeting the male specific lethal complex to chromatin in both humans and flies by binding to H4K20Me(1). PMID: 20943666
  2. A DNA-dependent interaction of MSL3 chromodomain with the H4 lysine20 monomethyl mark, was discovered. PMID: 20657587
  3. Nucleic acid binding determinants in the N terminus of MSL3 form an integrated domain which is required for interaction with MSL1 and localisation to X-chromosome. PMID: 15988010
  4. Localization to the male X chromosome and transcriptional upregulation of X-linked genes are two separable functions of the MSL-3 protein. PMID: 16547465
  5. These results provide strong evidence for both sequence-dependent and -independent steps in MSL targeting of dosage compensation to the male X chromosome. PMID: 18724933
  6. Using ChIP-chip analysis, the authors find that MSL3 chromodomain mutants retain binding to chromatin entry sites but show a clear disruption in the full pattern of MSL targeting in vivo, consistent with a loss of spreading. PMID: 19029895

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Subcellular Location
Nucleus. Chromosome. Note=Msl-3 is associated with hundreds of discrete sites along the length of the X chromosome in males and not in females, and is also associated with 10-20 autosomal sites in males.
Database Links

KEGG: dme:Dmel_CG8631

STRING: 7227.FBpp0076635

UniGene: Dm.2741

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