MSL8 Antibody

Code CSB-PA520529XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MSL8 Polyclonal antibody
Uniprot No.
Target Names
MSL8
Alternative Names
MSL8 antibody; At2g17010 antibody; F6P23.17 antibody; Mechanosensitive ion channel protein 8 antibody; Mechanosensitive channel of small conductance-like 8 antibody; MscS-Like protein 8 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana MSL8 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. Exhibits a 6.3-fold preference for chloride over sodium. Regulates osmotic forces during pollen hydration and germination.
Gene References into Functions
  1. An intact MSL8 channel is required for normal pollen function during hydration and germination. These data establish MSL8 as the first plant MS channel to fulfill previously established criteria for assignment as a mechanotransducer. PMID: 28339550
  2. MSL8 thus senses and responds to changes in membrane tension associated with pollen hydration and germination. PMID: 26494758
Subcellular Location
Cell membrane; Multi-pass membrane protein. Endomembrane system; Multi-pass membrane protein.
Protein Families
MscS (TC 1.A.23) family
Tissue Specificity
Expressed in tricellular and mature pollen, and in germinating tube. Not detected in leaves or roots.
Database Links

KEGG: ath:AT2G17010

STRING: 3702.AT2G17010.1

UniGene: At.40204

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