mTerf5 Antibody

Code CSB-PA156417XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) mTerf5 Polyclonal antibody
Uniprot No.
Target Names
mTerf5
Alternative Names
mTerf5 antibody; CG7175 antibody; Transcription termination factor 5 antibody; mitochondrial antibody; Mitochondrial transcription termination factor 5 antibody; mTerf5 antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) mTerf5 protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Binds promoter DNA and regulates initiation of transcription. Regulates mitochondrial replication and transcription. Required for normal topology and maintenance of mitochondrial DNA (mtDNA) levels. Regulates mtDNA replication by re-activating replication after replication pausing. Likely to regulate replication pausing by coordinating with the mitochondrial termination factor mTTF which promotes replication pausing. Their function in replication pausing prevents unregulated replication that may occur for example by collisions between the machineries of DNA replication and transcription during mtDNA synthesis. This ensures the incorporation of RNA transcripts into replication intermediates at the replication fork and allows for proper fork progression. Possibly functions downstream of Dref which activates genes involved in mtDNA replication and maintenance.
Gene References into Functions
  1. The authors propose that the two proteins participate in the transcription termination process, with D-MTERF5 engaged in relieving the block exerted by DmTTF. PMID: 22784680
Subcellular Location
Mitochondrion.
Protein Families
MTERF family
Database Links

KEGG: dme:Dmel_CG7175

STRING: 7227.FBpp0083029

UniGene: Dm.3719

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