Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans mtm-1 Polyclonal antibody

Uniprot No.

Q9N589

Target Names

mtm-1

Alternative Names

mtm-1 antibody; Y110A7A.5 antibody; Myotubularin-related protein 1 antibody; Phosphatidylinositol-3,5-bisphosphate 3-phosphatase antibody; EC 3.1.3.95 antibody; Phosphatidylinositol-3-phosphate phosphatase antibody; EC 3.1.3.64 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans mtm-1 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Dephosphorylates phosphatidylinositol 3-phosphate (PI3P) and phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2). Negatively regulates accumulation of PI3P on intracellular vesicles. Negatively regulates phagocytosis of apoptotic cells probably by limiting the recruitment and/or the activation of ced-5, ced-2 and ced-12 complex. In addition, may positively regulate phagosome maturation by promoting recycling of apoptotic receptor ced-1 back to the plasma membrane. Essential for embryonic and larval development. May promote migration of distal tip cells.

Gene References into Functions

MTM-1, a PI 3-phosphatase that antagonizes the activities of PIKI-1 and VPS-34 by down-regulating phosphatidylinositol 3-phosphate on phagosomes. PMID: 22272187
Loss of mtm-1 function leads to substantially reduced numbers of persistent cell corpses in engulfment mutants, which is a result of a restoration of engulfment function rather than of impaired or delayed programmed cell death. PMID: 21490059
Identified Caenorhabditis elegans myotubularin MTM-1 as a negative regulator of cell corpse engulfment. Show that loss of mtm-1 function accelerates the clearance of cell corpses by promoting their internalization. PMID: 19816564
at least three C. elegans MTMs play essential roles in coelomocyte endocytosis PMID: 12788949

Subcellular Location

Cell membrane; Peripheral membrane protein. Cell projection, phagocytic cup. Apical cell membrane; Peripheral membrane protein. Cytoplasmic granule membrane.

Protein Families

Protein-tyrosine phosphatase family, Non-receptor class myotubularin subfamily

Tissue Specificity

Expressed in embryo, larva and in adults. Expressed in a few head and tail neurons. Expressed in hypodermis, body wall and pharyngeal muscles, sheath cells, vulva, distal tip cells and coelomocytes.

Database Links

KEGG: cel:CELE_Y110A7A.5

STRING: 6239.Y110A7A.5

UniGene: Cel.7751

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mtm-1 Antibody

Product Details

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Target Background