Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) mtnN Polyclonal antibody

Uniprot No.

P0AF12

Target Names

mtnN

Alternative Names

mtnN antibody; mtn antibody; pfs antibody; yadA antibody; b0159 antibody; JW0155 antibody; 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase antibody; MTA/SAH nucleosidase antibody; MTAN antibody; EC 3.2.2.9 antibody; 5'-deoxyadenosine nucleosidase antibody; DOA nucleosidase antibody; dAdo nucleosidase antibody; EC 3.2.2.- antibody; 5'-methylthioadenosine nucleosidase antibody; MTA nucleosidase antibody; P46 antibody; S-adenosylhomocysteine nucleosidase antibody; AdoHcy nucleosidase antibody; SAH nucleosidase antibody; SRH nucleosidase antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) mtnN protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively. Also cleaves 5'-deoxyadenosine, a toxic by-product of radical S-adenosylmethionine (SAM) enzymes, into 5-deoxyribose and adenine. Thus, is required for in vivo function of the radical SAM enzymes biotin synthase and lipoic acid synthase, that are inhibited by 5'-deoxyadenosine accumulation. Can also use 5'-isobutylthioadenosine, 5'-n-butylthioadenosine, S-adenosyl-D-homocysteine, decarboxylated adenosylhomocysteine, deaminated adenosylhomocysteine and S-2-aza-adenosylhomocysteine as substrates in vitro.

Gene References into Functions

The transition-state analogue affinity and thermodynamics in mutant VcMTAN became even more unlike those of EcMTAN, the opposite of expectations from catalytic site similarity PMID: 25806409
transition state structure investigated using multiple kinetic isotope effects and its geometric and electrostatic properties obtained using density functional methods PMID: 16128565
Pfs transcription is shown to rely on both sigma 70 and sigma 38 (rpoS), and the latter is subject to induction that increases pfs expression PMID: 16950920

Protein Families

PNP/UDP phosphorylase family, MtnN subfamily

Database Links

KEGG: ecj:JW0155

STRING: 316385.ECDH10B_0139

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mtnN Antibody

Product Details

Customer Reviews and Q&A

Target Background