MTR4 Antibody

Code CSB-PA344029XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MTR4 Polyclonal antibody
Uniprot No.
Target Names
MTR4
Alternative Names
MTR4 antibody; DOB1 antibody; YJL050W antibody; J1158 antibody; ATP-dependent RNA helicase DOB1 antibody; EC 3.6.4.13 antibody; mRNA transport regulator MTR4 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MTR4 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
ATP-dependent RNA helicase required for the 3'-end formation of 5.8S RNA. Cofactor for the exosome complex that unwinds secondary structure in pre-rRNA. Required for nucleocytoplasmic transport of mRNA. May serve as a chaperone which translocates or normalizes the structure of mRNAs in preparation for export. Component of the TRAMP complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism limiting inappropriate expression of genetic information. Polyadenylation is required for the degradative activity of the exosome on several of its nuclear RNA substrates.
Gene References into Functions
  1. Mpp6-exosomes can recruit Mtr4, while Mpp6 and Rrp47 each contribute to Mtr4-dependent RNA decay, with maximal Mtr4-dependent decay observed with both cofactors. PMID: 28742025
  2. report the 3.2 A resolution crystal structure of S. cerevisiae Mtr4 bound to the interacting region of Nop53, revealing how the KOW domain of the helicase recognizes the AIM sequence of Nop53 with a network of hydrophobic and electrostatic interactions PMID: 28883156
  3. Mtr4p structural domain mutants in the helicase core reduced Mtr4p RNA binding, ATPase activity and unwinding activity in vitro. PMID: 26820724
  4. Mtr4 is linked to the core exosome through its binding to the Rrp6 - Rrp47 composite surface. PMID: 25319414
  5. The studt demonstrates that the ratchet helix modulates helicase activity and suggest that the arch domain plays a previously unrecognized role in unwinding substrates. PMID: 25414331
  6. The RNA binding part of the Mtr4p arch, the KOW domain, as the essential component for TRAMP-mediated exosome activation. PMID: 22402490
  7. Data establish Mtr4p as a critical regulator of polyadenylation by TRAMP and reveal that an RNA helicase can control the activity of another enzyme in a highly complex fashion and in response to features in RNA. PMID: 21663793
  8. Unique properties of the Mtr4-polyA interaction indicate that one role of Mtr4 is to discriminate between substrates and thereby maintain contact with the short polyadenylated sequences that signal degradation. PMID: 21058657
  9. Data show that Mtr4 has evolved specific structural and surface features to perform its multiple functions. PMID: 20566885
  10. The crystal structure reveals a new arch-like domain that is specific to Mtr4 and Ski2 (the cytosolic homologue of Mtr4) and is required for rRNA processing. PMID: 20512111
  11. Data suggest that neither the poly(A) polymerase activity of Trf4 nor the helicase activity of Mtr4 plays a role in the enhancement of RNA degradation by the nuclear exosome component Rrp6. PMID: 19955569
  12. Mtr4p displays a marked preference for binding to poly(A) RNA relative to an oligoribonucleotide of the same length and a random sequence. PMID: 18096702

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Subcellular Location
Nucleus.
Protein Families
Helicase family, SKI2 subfamily
Database Links

KEGG: sce:YJL050W

STRING: 4932.YJL050W

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