Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) mTTF Polyclonal antibody

Uniprot No.

Q9V3F3

Target Names

mTTF

Alternative Names

mTTF antibody; CG18124 antibody; Transcription termination factor antibody; mitochondrial antibody; Mitochondrial transcription termination factor antibody; mTTF antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) mTTF protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Transcription termination factor. Binds promoter DNA and regulates mitochondrial replication and transcription. Transcription termination activity may be polarized with highest termination activity occurring when its DNA-binding site is positioned in the reverse orientation with respect to the incoming RNA polymerase. Required for normal topology and maintenance of mitochondrial DNA (mtDNA) levels. Regulates mtDNA replication by promoting replication pausing, possibly by acting as a natural barrier to replication fork progression. Its function in replication pausing prevents unregulated replication that may occur for example by collisions between the machineries of DNA replication and transcription during mtDNA synthesis. This ensures the incorporation of RNA transcripts into replication intermediates at the replication fork and allow for proper fork progression. Shares mtDNA binding sites with the mitochondrial termination factor mTerf5 and thereby may antagonize mTerf5 function during replication to regulate pausing. Likely to function downstream of Dref which activates genes involved in mtDNA replication and maintenance.

Gene References into Functions

mTerf5 knockdown led to enhanced replication pausing at mTTF binding sites, a decrease in fragile replication intermediates containing single-stranded segments, and the disappearance of species containing segments of RNA/DNA hybrid PMID: 24068965
The first ever-obtained evidence for an in vivo role of an animal mitochondrial transcription termination factor. PMID: 16648357
DmTTF might play multiple roles in the mtDNA transcription process, for which different levels of the protein with respect to mtRNApol are required. PMID: 19032147

Subcellular Location

Mitochondrion.

Protein Families

MTERF family

Database Links

KEGG: dme:Dmel_CG18124

STRING: 7227.FBpp0080213

UniGene: Dm.5596

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mTTF Antibody

Product Details

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Target Background