mukB Antibody

1. Study reports that MukB binds topologically to DNA in vitro, and this binding is preferentially single-stranded DNA (ssDNA) rather than double-stranded DNA. The binding of MukB to ssDNA does not require ATP but the thermal energy enhances the binding. PMID: 27387439
2. Cells carrying a mukB mutant allele that encodes a protein that does not interact with topoisomerase IV exhibit severe nucleoid decompaction leading to chromosome segregation defects. These findings suggest that the MukB-topoisomerase IV complex may provide a scaffold for DNA condensation. PMID: 28842485
3. MukB hinge region binds DNA and that this region of the protein is involved in sequestration of supercoils. Cells carrying mutations in the MukB hinge that reduce DNA condensation in vitro exhibit nucleoid decondensation in vivo. PMID: 28842486
4. MukB-ParC interaction may play a role in chromosome organization rather than in separation of daughter chromosomes PMID: 23349462
5. The positioning of replication forks is affected by the disruption of the mukB gene. PMID: 16133165
6. MukB has a unique set of topological properties, compared with other SMC proteins, and is likely to exist in two different conformations PMID: 16368697
7. Stable association of MukB with the chromosome required MukEF. PMID: 17644586
8. In mukB mutant cells, the two chromosome arms do not separate into distinct cell halves, but extend from pole to pole with the oriC region located at the old pole. PMID: 17824928
9. Using these experimental constraints, phylogenetic data, and coiled-coil prediction algorithms, the authors propose a pairing scheme for the discontinuous segments in the coiled coil. PMID: 19482037

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KEGG: ecj:JW0907

STRING: 316385.ECDH10B_0994