murA Antibody

Code CSB-PA363951XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) murA Polyclonal antibody
Uniprot No.
Target Names
murA
Alternative Names
murA antibody; murZ antibody; b3189 antibody; JW3156UDP-N-acetylglucosamine 1-carboxyvinyltransferase antibody; EC 2.5.1.7 antibody; Enoylpyruvate transferase antibody; UDP-N-acetylglucosamine enolpyruvyl transferase antibody; EPT antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) murA protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine. Target for the antibiotic fosfomycin.
Gene References into Functions
  1. The lysis protein A2 of enterobacteria phage Qbeta preferentially binds bacterial MurA liganded with UDP-NAG, thereby preventing catalysis by occluding PEP from accessing the active site. PMID: 22934834
  2. The kinetics of MurA-catalyzed tetrahedral intermediate partitioning provides functional evidence of the relevance of MurA's conformationally restricted active site as part of a choreographed product dissociation process. PMID: 19899805
  3. MurA catalyzed the formation of a covalent adduct between its inhibitor, cnicin and its substrate UDP-N-acetylglucosamine. PMID: 18672863
Subcellular Location
Cytoplasm.
Protein Families
EPSP synthase family, MurA subfamily
Database Links

KEGG: ecj:JW3156

STRING: 316407.85675983

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