murD Antibody

Code CSB-PA318612XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) murD Polyclonal antibody
Uniprot No.
Target Names
murD
Alternative Names
murD antibody; b0088 antibody; JW0086 antibody; UDP-N-acetylmuramoylalanine--D-glutamate ligase antibody; EC 6.3.2.9 antibody; D-glutamic acid-adding enzyme antibody; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) murD protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA).
Gene References into Functions
  1. A detailed analysis provides insight into the events that lead to the closure of MurD and reveals that minor structural modifications contribute to major overall conformation alterations. PMID: 27031227
  2. Results report the high resolution crystal structures of MurD in complexes with two novel inhibitors designed to mimic the transition state of the reaction, which contain either the D-Glu or the L-Glu moiety. PMID: 17507028
  3. Evaluation of three possible reaction pathways leading to tetrahedral intermediate formation by MurD ligase. PMID: 18704940
Subcellular Location
Cytoplasm.
Protein Families
MurCDEF family
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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