Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) Myo31DF Polyclonal antibody

Uniprot No.

Q23978

Target Names

Myo31DF

Alternative Names

Myo31DF antibody; Myo1D antibody; CG7438 antibody; Unconventional myosin ID antibody; MyoID antibody; Brush border myosin IA antibody; BBMIA antibody; Myosin 1D antibody; Myo1D antibody; Myosin-IA antibody; MIA antibody; MyoIA antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) Myo31DF protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Unconventional myosin that functions as actin-based motor protein with ATPase activity. Binds to membranes enriched in phosphatidylinositol 4-5-bisphosphate, and can glide along actin filaments when anchored to a lipid bilayer. Generates left-right asymmetry at the level of single cells, organs and the whole body via its interaction with the actin cytoskeleton, both in the embryo and the adult. Normal left-right asymmetry of the larval midgut and hindgut requires expression in the embryonic hindgut epithelium during a critical time period, 10 to 12.75 hours after egg laying. This period corresponds to a late stage of germband retraction, and precedes left-right asymmetric morphogenesis. Expression in segment H1 of the imaginal ring is required at 0 to 24 hours after pupation for changes of cell shape and orientation in the H2 segment, which then gives rise to normal, dextral looping of the adult hindgut. Required during a critical period, 126-132 hours after egg laying, for normal, dextral rotation of the adult male genitalia. Has a double role by promoting dextral rotation in the posterior compartment of segment A8 of the male genital disk, and in repressing sinistral looping in the anterior compartment.

Gene References into Functions

Myo31DF-overexpressing cells suggest that cell-shape chirality was established in each cell and reflects intrinsic planar cell chirality. PMID: 24800645
DE-Cadherin regulates unconventional Myosin ID and Myosin IC in Drosophila left-right asymmetry establishment. PMID: 22491943
left-right(LR)directional rotation of hindgut epithelial tube; DECad is distributed to cell boundaries with LR asymmetry which is responsible for planar cell-shape chirality formation; myosin ID switches LR polarity found in PCC and in DE-Cad distribution PMID: 21764746
the actin cytoskeleton and myosin I proteins may be crucial for generating left-right asymmetry in invertebrates PMID: 16598258
Myo31DF interacts and colocalizes with beta-catenin, suggesting that situs inversus genes can direct left-right development through the adherens junction PMID: 16598259
the organ specificities of the Myo31DF and Myo61F activities depended on their head regions PMID: 18521948

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Subcellular Location

Cytoplasm. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction. Cell junction, adherens junction. Cell projection.

Protein Families

TRAFAC class myosin-kinesin ATPase superfamily, Myosin family

Tissue Specificity

In the embryo, expressed in gastric caeca, midgut cells of the proventriculus, and in the mid and hindgut. In the larval gut brush border, expression is in the terminal web domain. In the adult gut brush border, expression remains in the web domain and ha

Database Links

KEGG: dme:Dmel_CG7438

STRING: 7227.FBpp0079625

UniGene: Dm.5426

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Myo31DF Antibody

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