MYB30 Antibody

Code CSB-PA825103XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MYB30 Polyclonal antibody
Uniprot No.
Target Names
MYB30
Alternative Names
MYB30 antibody; hsr1 antibody; At3g28910 antibody; MLD15.8 antibody; Transcription factor MYB30 antibody; Myb-related protein 30 antibody; AtMYB30 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana MYB30 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription factor that binds specifically to the DNA sequence 5'-AACAAAC-3'. Acts as a positive regulator of hypersensitive cell death. Acts as a positive regulator of salicylic acid synthesis. Regulates very-long-chain fatty acid biosynthesis. Acts cooperatively with BZR2 to promote expression of a subset of brassinosteroids target genes. Transcriptional activity and hypersensitive response control negatively regulated by PLA2-ALPHA. Involved in the regulation of abscisic acid (ABA) signaling. Increased levels of MYB30 can accelerate flowering both in long and short days through the regulation of FT.
Gene References into Functions
  1. showed that MYB30 is necessary for root growth regulation during defense responses PMID: 29712840
  2. MYB30 regulates oxidative and heat stress responses through calcium signaling, which is at least partially mediated by ANN1 and ANN4. PMID: 28726305
  3. MYB30-INTERACTING E3 LIGASE 1 (MIEL1) E3 ubiquitin ligase controls the protein stability of the two MYB transcription factors, MYB96 and MYB30, and thereby wax biosynthesis in Arabidopsis. These observations indicate that the MIEL1-MYB96 module is important for balanced cuticular wax biosynthesis in developing inflorescence stems. PMID: 28838126
  4. The SBT5.2 gene produces two distinct transcripts encoding a canonical secreted subtilase [SBT5.2(a)] and an intracellular protein [SBT5.2(b)]. SBT5.2(b) localizes to endosomes where it interacts with and retains the defence-related transcription factor MYB30. PMID: 27685353
  5. Elevated levels of MYB30 in the phloem accelerate flowering in Arabidopsis through the regulation of FLOWERING LOCUS T. PMID: 24587042
  6. S-nitrosylation influences the structure and DNA binding activity of MYB30 from Arabidopsis thaliana. PMID: 24583075
  7. MIEL1 (MYB30-Interacting E3 Ligase1), an Arabidopsis RING-type E3 ubiquitin ligase that interacts with and ubiquitinates MYB30, leads to MYB30 proteasomal degradation and downregulation of its transcriptional activity. PMID: 23403577
  8. coordination of ABI5 and MYB30 sumoylation by SIZ1 may balance gene expression, which is required for regulation of ABA signaling during seed germination. PMID: 22814374
  9. Results suggest that RNA silencing directly mediates downregulation of AtMYB30 expression, both in young seedlings and in adult plants. PMID: 20605724
  10. BES1 and AtMYB30 function cooperatively to promote brassinosteroid target gene expression. PMID: 19170933

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in vascular tissues of leaves, hypocotyl and roots.
Database Links

KEGG: ath:AT3G28910

STRING: 3702.AT3G28910.1

UniGene: At.10902

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