MYB44 Antibody

Code CSB-PA863724XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MYB44 Polyclonal antibody
Uniprot No.
Target Names
MYB44
Alternative Names
MYB44 antibody; MYBR1 antibody; At5g67300 antibody; K8K14.2 antibody; Transcription factor MYB44 antibody; Myb-related protein 44 antibody; AtMYB44 antibody; Myb-related protein R1 antibody; AtMYBR1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana MYB44 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription factor. Represses the expression of protein phosphatases 2C in response to abscisic acid (ABA). Confers resistance to abiotic stresses dependent of ABA. In response to auxin, activates the transcription of the auxin-responsive gene IAA19. The IAA19 transcription activation by MYB44 is enhanced by direct interaction between MYB44 and PYL8. Transcriptional activator of WRKY70 by direct binding to its promoter region, especially at 5'-TAACNG-3' and 5'-CNGTTA-3' symmetric motifs. Activates salicylic acid (SA)- mediated defenses and subsequent resistance to biotrophic pathogen P.syringae pv. tomato DC3000, but represses jasmonic acid (JA)-mediated defenses responses against the necrotrophic pathogen A.brassicicola.
Gene References into Functions
  1. salt stress induces the eviction of H2A.Z-containing nucleosomes from the AtMYB44 promoter region. PMID: 29649476
  2. AtMYB44 might participate in the 3OC6-HSL-mediated primary root growth via regulating the expression of cytokinin- and auxin-related genes. The data establish a genetic connection between the regulatory role of AtMYB44 in phytohormones-related gene expression and plant response to the bacterial QS signal. PMID: 27604593
  3. The authors herein revealed that the MYB44-mediated prevention of Pseudomonas syringae penetration through the stomata is one of the components responsible for Penicillium simplicissimum GP17-2-elicited induced systemic resistance. PMID: 27301421
  4. Dominant repression by Arabidopsis transcription factor MYB44 causes oxidative damage and hypersensitivity to abiotic stress. PMID: 24531138
  5. AtMYB44 is an integrator of cross-talk between salicylic acid and jasmonic acid in plant defense responses. PMID: 23603962
  6. the C-terminal side of the protein, but not the N-terminal side of the R2R3 domain, contributes to the functional activity and specificity of AtMYB44 through interactions with other regulators generated by each of a variety of stimuli. PMID: 23161171
  7. the transcriptional regulator MYB44 has a role in the control of seed germination in Arabidopsis thaliana. PMID: 22704933
  8. AtMYB44 gene activation appears to not be induced by any specific hormone PMID: 20016937
  9. Enhanced abiotic stress tolerance of transgenic Arabidopsis overexpressing AtMYB44 was conferred by reduced expression of genes encoding a group of serine/threonine protein phosphatases 2C. PMID: 18162593

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in roots, stems, leaves, inflorescence, and flowers (including stamen, floral nectar, carpel, petal and sepal), mostly in vasculatures and stomata.
Database Links

KEGG: ath:AT5G67300

STRING: 3702.AT5G67300.1

UniGene: At.252

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