MYB65 Antibody

Code CSB-PA962389XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MYB65 Polyclonal antibody
Uniprot No.
Target Names
MYB65
Alternative Names
MYB65 antibody; At3g11440 antibody; F24K9.11 antibody; Transcription factor MYB65 antibody; Myb-related protein 65 antibody; AtMYB65 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana MYB65 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcriptional activator of alpha-amylase expression that binds to 5'-CAACTGTC-3' motif in target gene promoter. In vegetative tissues, inhibits growth by reducing cell proliferation. Promotes the expression of aleurone-related genes (e.g. CP1, CP, GASA1, BXL1 and BXL2) in seeds. Together with MYB33 and MYB101, promotes the programmed cell death (PCD) the vacuolation of protein storage vacuoles (PSVs) in the aleurone layers during seed germination. Together with MYB33, facilitates anther and tapetum development.
Gene References into Functions
  1. MiR159a and miR159b appear to continuously repress MYB33 and MYB65 expression to levels that have no major impact on rosette development. PMID: 27542984
  2. There may be factors, in addition to complementarity, that control plant miRNA efficacy, such factors appear to be optimised in regards to the endogenous and ancient miR159:MYB33/65 relationship. PMID: 24103298
  3. MYB33 and MYB65 play no part in gibberellin-mediated growth or flowering in vegetative tissues. PMID: 20699403
  4. Consistent with functional redundancy, promoter-GUS fusions of MYB33 and MYB65 gave identical expression patterns in flowers (sepals, style, receptacle, anther filaments, and connective but not in anthers themselves), shoot apices, and root tips. PMID: 15722475
Subcellular Location
Nucleus.
Tissue Specificity
Mostly expressed in roots (e.g. root tips), stems, pollen, shoot apices, flowers and floral shoot tips, and, to a lower extent, in leaves and siliques.
Database Links

KEGG: ath:AT3G11440

STRING: 3702.AT3G11440.1

UniGene: At.43844

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