XI-K Antibody

Code CSB-PA242456XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) XI-K Polyclonal antibody
Uniprot No.
Target Names
XI-K
Alternative Names
XI-K antibody; XIK antibody; At5g20490 antibody; F7C8.80 antibody; Myosin-17 antibody; Myosin XI K antibody; AtXIK antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana XI-K protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Myosin heavy chain that is required for the cell cycle-regulated transport of various organelles and proteins for their segregation. Functions by binding with its tail domain to receptor proteins on organelles and exerting force with its N-terminal motor domain against actin filaments, thereby transporting its cargo along polarized actin cables. Involved in the tip growth of root hair cells and in the elongation of trichome stalk and branches. Plays a major role in trafficking of Golgi stacks, mitochondria and peroxisomes during root hair development. Acts as the primary contributor to ER streaming with a major role in the movement of Golgi bodies. Required for development of pavement cells, trichomes, and stigmatic papillae.
Gene References into Functions
  1. By performing coexpression studies with AtDCP1, dominant-negative myosin fragments, as well as functional full-length myosin XI-K, the association of P-bodies and myosins was analyzed in detail. PMID: 24525673
  2. The class XI myosins XI-K, XI-1 and XI-2 have partially redundant roles in the growth of shoot epidermis. Myosin XI-K plays more important role whereas myosins XI-1 and XI-2 have minor roles in the determination of size and shape of epidermal cells. PMID: 22672737
  3. Data suggest that the myosin XIK is required for normal actin dynamics and plays a role in the subapical region of growing root hairs to facilitate optimal growth. PMID: 24116145
  4. a tail fragment of myosin XIK could inhibit the movement of several post-Golgi organelles, such as the trans-Golgi network, pre-vacuolar compartment, and endosomes, as well as total cytoplasmic streaming PMID: 21914656
  5. knockouts exhibited defects in root hair elongation suggesting that the myosin-driven motility plays a significant role in a polar tip growth PMID: 18178669
  6. Arabidopsis myosins XIE, and XIK, are involved in Golgi, peroxisome, and mitochondrial movement. PMID: 18503043

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Subcellular Location
Cytoplasm. Note=Colocalizes with peroxisome, cytoplasmic vesicles, endomembrane vesicles, endoplasmic reticulum and/or organelles.
Protein Families
TRAFAC class myosin-kinesin ATPase superfamily, Myosin family, Plant myosin class XI subfamily
Tissue Specificity
Expressed ubiquitously.
Database Links

KEGG: ath:AT5G20490

STRING: 3702.AT5G20490.1

UniGene: At.43864

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