myo2 Antibody

Datasheet

Code	CSB-PA890796XA01SXV
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Product Details

Full Product Name

Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) myo2 Polyclonal antibody

Uniprot No.

Q9USI6

Target Names

myo2

Alternative Names

myo2 antibody; SPCC645.05c antibody; Myosin type-2 heavy chain 1 antibody; Myosin type II heavy chain 1 antibody

Raised in

Rabbit

Species Reactivity

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Immunogen

Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) myo2 protein

Immunogen Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Required for cell division. It is a component of the cdc12 'spot', a structure thought to mark the site of septation. May work in conjunction with myo3.

Gene References into Functions

Myo2p may function in two different and essential modes during cytokinesis: a motor activity-independent form that can promote actomyosin ring assembly and a motor activity-dependent form that supports ring contraction. PMID: 28238661
Myo2 role in the Cdc12-associated actin filaments actomyosin ring assembly. PMID: 28951543
Three myosins function in a hierarchal, complementary manner to accomplish cytokinesis, with Myo2 and Myo51 taking the lead during contractile ring assembly and Myp2 making the greatest contribution to constriction. PMID: 26144970
Data show that contractile ring assembly is delayed in Myo2p motor and tropomyosin mutants, but occurs prematurely in cells engineered to express two copies of myo2. PMID: 20110347
Results describe the role of regulatory light-chain (Rlc1p) and heavy-chain phosphorylation in controlling fission yeast myosin-II (Myo2p) motor activity and function during cytokinesis. PMID: 19570908

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Protein Families

TRAFAC class myosin-kinesin ATPase superfamily, Myosin family

Database Links

KEGG: spo:SPCC645.05c

STRING: 4896.SPCC645.05c.1

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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myo2 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background