Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MYO4 Polyclonal antibody

Uniprot No.

P32492

Target Names

MYO4

Alternative Names

MYO4 antibody; SHE1 antibody; YAL029C antibody; FUN22 antibody; Myosin-4 antibody; SWI5-dependent HO expression protein 1 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MYO4 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud and in the daughter cell. Recruited to specific mRNAs including the ASH1 mRNA, coding for a repressor of the HO endonuclease, via its interaction with SHE3.

Gene References into Functions

Data suggest a model in which simultaneous interactions between the microtubule and dynein enables myosin 4 (She1) to directly affect dynein motility. PMID: 29247176
Results indicate that inhibition of dynein activity by She1 is important to prevent excessive detachment of cytoplasmic microtubules, particularly in G1 cells. PMID: 22535527
multiple, nonprocessive Myo4 motors can generate continuous transport of mRNA to the bud tip PMID: 20457760
In Saccharomyces cerevisiae, the MyoV motor Myo4p interacts with She3p to localize asymmetric synthesis of HO 1 (ASH1) mRNA into the bud of dividing cells PMID: 20439999
anchoring of ASH1 mRNA requires molecular remodeling of the Myo4p-She3p-She2p heterotrimeric complex, a process that is apparently altered when mRNA is artificially tethered to She2p. PMID: 15328357
These results suggest that the oligomeric state of Myo4p is important for its motility and ability to transport mRNA. PMID: 17893244
continuous transport of mRNA by Myo4p-She3p in yeast is accomplished either by multiple high duty cycle monomers or by molecules that may be dimerized by She2p, the homodimeric downstream binding partner of She3p PMID: 18175803
The most important feature for correct localization is the retention of coupling between all the members of the complex (Myo4p-She3p-She2p-ASH1 mRNA), which is aided by She3p being a tightly bound subunit of Myo4p. PMID: 19477930

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Subcellular Location

Bud. Note=Accumulates preferentially in growing buds.

Protein Families

TRAFAC class myosin-kinesin ATPase superfamily, Myosin family

Database Links

KEGG: sce:YAL029C

STRING: 4932.YAL029C

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MYO4 Antibody

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