jar Antibody

Code CSB-PA958827XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) jar Polyclonal antibody
Uniprot No.
Target Names
jar
Alternative Names
jar antibody; Mhc95F antibody; CG5695 antibody; Myosin heavy chain 95F antibody; 95F MHC antibody; Protein jaguar antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) jar protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Myosin is a protein that binds to actin and has ATPase activity that is activated by actin. Together CLIP-190 and jar may coordinate the interaction between the actin and microtubule cytoskeleton. May link endocytic vesicles to microtubules and may be involved in transport in the early embryo and in the dynamic process of dorsal closure. It is believed that its function changes during the life cycle.
Gene References into Functions
  1. These observations support the notion that a major function of Myosin VI in the nerve terminal is tethering synaptic vesicles to proper sub-cellular location within the bouton PMID: 25062032
  2. These data demonstrate that generating an organized and functional actin structure in this cell requires multiple activities coordinated by myosin VI. PMID: 21853045
  3. The androcam structure and its binding to the myosin VI structural (Insert 2) and regulatory (IQ) light chain sites are distinct from those of calmodulin. PMID: 22851764
  4. Myosin VI is crucial for correct cell morphology and maintenance of adhesive cellular contacts within epithelial cell layers. PMID: 15454264
  5. Myosin VI stabilizes a branched actin network in actin structures (cones) that mediate the separation of the syncytial spermatids. I PMID: 16571671
  6. The spatial and temporal expression of Myosin VI was examined by expressing a Green Fluorescent Protein tagged Myosin VI molecule, under the control of a Myosin VI-Gal4 line. PMID: 17185045
  7. Genetic characterization of the Drosophila jaguar322 mutant reveals that complete myosin VI loss of function is not lethal. PMID: 18493084
  8. Results suggest that myosin VI does not need to dimerize via the predicted coiled coil to stabilize actin in vivo. PMID: 19005209

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Subcellular Location
Cytoplasm, cytoskeleton. Note=Microtubule-associated.
Protein Families
TRAFAC class myosin-kinesin ATPase superfamily, Myosin family
Tissue Specificity
Isoform B is present at a higher level in the head and gonads than in the thoraxes. Isoform 145 kDa is found only in the head. CLIP-190 and jar are coexpressed at several times in development and in a number of tissues, including embryonic axonal neuron p
Database Links

KEGG: dme:Dmel_CG5695

STRING: 7227.FBpp0290582

UniGene: Dm.7884

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