nagC Antibody

Code CSB-PA365237XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) nagC Polyclonal antibody
Uniprot No.
Target Names
nagC
Alternative Names
nagC antibody; nagR antibody; b0676 antibody; JW0662 antibody; N-acetylglucosamine repressor antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) nagC protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Acts as a repressor of the nagEBACD operon and acts both as an activator and a repressor for the transcription of the glmSU operon.
Gene References into Functions
  1. This work identifies four amino acids in the linker region which allow NagC to distinguish between a C/G and an A/T bp at positions +/-11 of the operators. PMID: 25452338
  2. Mutations in NagC, at positions which form a structural motif resembling a glucose binding site in Mlc, produce permanently repressing forms of NagC, suggesting that this motif forms a N-acetylglucosamine 6-phosphate binding site in NagC. PMID: 18067539
  3. Results show that changes at either of two Mlc operators upstream of ptsG are sufficient to lose regulation by Mlc but that both types of changes in both operators are necessary to convert ptsG to a gene regulated by NagC. PMID: 18469102
  4. NagC binds to a single, high-affinity site overlapping the promoter of galP. PMID: 19007420
Protein Families
ROK (NagC/XylR) family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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