NHA1 Antibody

Code CSB-PA860755XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) NHA1 Polyclonal antibody
Uniprot No.
Target Names
NHA1
Alternative Names
NHA1 antibody; YLR138W antibody; L3149 antibody; L9606.4 antibody; Na(+)/H(+) antiporter antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) NHA1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Sodium export from cell, takes up external protons in exchange for internal sodium ions.
Gene References into Functions
  1. Erv14 cargo receptor participates in yeast salt tolerance via its interaction with the plasma-membrane Nha1 cation/proton antiporter. PMID: 26440927
  2. All three potassium-exporting systems the Nha1 antiporter, Ena ATPase and Tok1 channel contribute to potassium homeostasis and are active upon potassium limitation in wild-type cells. PMID: 22329368
  3. A detailed molecular mechanism for the Na+/H+ antiporter NHA1 is demonstrated, and the functional importance of domains proximal to the membrane region is discussed. PMID: 14999019
  4. analysis of Nha1p transport activity using isolated secretory vesicles PMID: 15950597
  5. results support the notion that Nha1p exists in membranes as a dimer and that the interaction of its monomers is important for its antiporter activity PMID: 16360116
  6. Nha1p and its C-terminus in the immediate response to hyperosmotic shock as part of the rapid rescue mechanism. PMID: 16402204
  7. The Na+,K+/H+ -antiporter Nha1 influences the plasma membrane potential of Saccharomyces cerevisiae. PMID: 16879429
  8. Yeast Kha1 proteins probably belong to the same subfamily as bacterial antiporters, whereas Nhal proteins form a distinct subfamily PMID: 17176761
  9. sphingolipids are required for the stable localization of Nha1p to the plasma membrane PMID: 19254924

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Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
Fungal Na(+)/H(+) exchanger family
Database Links

KEGG: sce:YLR138W

STRING: 4932.YLR138W

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