nanA Antibody

Code CSB-PA015994XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) nanA Polyclonal antibody
Uniprot No.
Target Names
nanA
Alternative Names
nanA antibody; npl antibody; b3225 antibody; JW3194 antibody; N-acetylneuraminate lyase antibody; NAL antibody; Neu5Ac lyase antibody; EC 4.1.3.3 antibody; N-acetylneuraminate pyruvate-lyase antibody; N-acetylneuraminic acid aldolase antibody; NALase antibody; Sialate lyase antibody; Sialic acid aldolase antibody; Sialic acid lyase antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) nanA protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the reversible aldol cleavage of N-acetylneuraminic acid (sialic acid; Neu5Ac) to form pyruvate and N-acetylmannosamine (ManNAc) via a Schiff base intermediate. Experiments show the true substrate is aceneuramate (linearized Neu5Ac), which forms spontaneously at alkaline pH. Linear aceneuramate can be provided by NanQ.
Gene References into Functions
  1. analysis of modulation of substrate specificities of D-sialic acid aldolase through single mutations of Val-251 PMID: 21270125
  2. A comparison of mutant and wild-type nanA structures, in combination with the specificity profiles of the variants, reveals subtle differences that explain the details of the substrate specificity changes. PMID: 20826162
  3. Results describe the structure of a mutant variant of Escherichia coli N-acetyl-d-neuraminic acid lyase (NAL), E192N, in complex with pyruvate in a new crystal form. PMID: 19923724
  4. the dimer-dimer interface of NAL is intolerant to mutation and essential for functional expression. PMID: 19646958
Subcellular Location
Cytoplasm.
Protein Families
DapA family, NanA subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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