narJ Antibody

Code CSB-PA365240XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) narJ Polyclonal antibody
Uniprot No.
Target Names
narJ
Alternative Names
narJ antibody; b1226 antibody; JW1217 antibody; Nitrate reductase molybdenum cofactor assembly chaperone NarJ antibody; Redox enzyme maturation protein NarJ antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) narJ protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Chaperone required for proper molybdenum cofactor insertion and final assembly of the membrane-bound respiratory nitrate reductase 1. Required for the insertion of the molybdenum into the apo-NarG subunit, maybe by keeping NarG in an appropriate competent-open conformation for the molybdenum cofactor insertion to occur. NarJ maintains the apoNarGH complex in a soluble state. Upon insertion of the molybdenum cofactor, NarJ seems to dissociate from the activated soluble NarGH complex, before its association with the NarI subunit on the membrane.
Gene References into Functions
  1. the NarJ chaperone interacts at two distinct binding sites of the apoenzyme PMID: 16286471
  2. The interactome of NarJ exhibits Tat-dependence. PMID: 16540088
  3. Data demonstrate that NarJ has a multifunctional role in nitrate reductase A biosynthesis, as it orchestrates both the maturation and the assembly steps. PMID: 17442677
Subcellular Location
Cytoplasm.
Protein Families
NarJ/NarW family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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