rg Antibody

Datasheet

Code	CSB-PA015482XA01DLU
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Target Background

Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) rg Polyclonal antibody

Uniprot No.

Q9W4E2

Target Names

Alternative Names

rg antibody; Akap550 antibody; CG44835 antibody; Neurobeachin antibody; A-kinase anchor protein 550 antibody; AKAP 550 antibody; Protein rugose antibody; dAKAP550 antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) rg protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Binds to type II regulatory subunits of protein kinase A and anchors/targets them to the membrane. May anchor the kinase to cytoskeletal and/or organelle-associated proteins. Required for correct retinal pattern formation and may function in cell fate determination through its interactions with the EGFR and Notch signaling pathways. Required for associative odor learning and short-term memory. Involved in development of the neuromuscular junction and the mushroom body.

Gene References into Functions

adult rg loss-of-function mutants exhibit defective social interactions, impaired habituation, aberrant locomotion, and hyperactivity. PMID: 26100104
This study demonistrated that functional homologies between Drosophila Rugose and mammalian NBEA that will help clarify the role of BEACH proteins in the development and function of the CNS in vivo. PMID: 23100440
Drosophila spoonbill encodes a dual-specificity A-kinase anchor protein essential for oogenesis. PMID: 21983075
results suggest that rg is required for correct retinal pattern formation and may function in cell fate determination through its interactions with the EGFR and Notch signaling pathways PMID: 12072466
results suggest that rugose gene encodes a secondary pigment cell specific function, and plays an essential role in determining the optic lobe's structure PMID: 14708865
Rugose (rg) plays an important role in the integration of different signals required for the exact regulation of cone cell development and survival. PMID: 15647755

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Subcellular Location

Cytoplasm. Membrane; Peripheral membrane protein. Perikaryon. Note=Found in the cortical region of perikarya with no expression in neuropil regions. Located at or near the Golgi network in perikarya of the dorsal medial cluster neurons.

Protein Families

WD repeat neurobeachin family

Tissue Specificity

In early embryos, ubiquitous expression with elevated levels in ventral furrow and flanking mesectodermal cells, neuroblasts and mesoderm. Late embryos show reduced expression in epidermis and skeletal muscle and elevated in nervous system, gut endotheliu

Database Links

KEGG: dme:Dmel_CG44835

STRING: 7227.FBpp0288527

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