NAC101 Antibody

Code CSB-PA237996XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NAC101 Polyclonal antibody
Uniprot No.
Target Names
NAC101
Alternative Names
NAC101 antibody; VND6 antibody; At5g62380 antibody; MMI9.21 antibody; NAC domain-containing protein 101 antibody; ANAC101 antibody; Protein VASCULAR RELATED NAC-DOMAIN 6 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana NAC101 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription activator that binds to the secondary wall NAC binding element (SNBE), 5'-(T/A)NN(C/T)(T/C/G)TNNNNNNNA(A/C)GN(A/C/T)(A/T)-3', and to the tracheary elements (TE) specific regulating cis-element (TERE), 5'-CTTNAAAGCNA-3', in the promoter of target genes (e.g. genes involved in secondary wall biosynthesis, cell wall modification such as xylan accumulation, and programmed cell death). Involved in xylem formation in roots and shoots, especially regulating metaxylem vessel differentiation by promoting immature xylem vessel-specific genes expression, especially genes regulating programmed cell death (PCD) and secondary wall formation in tracheary elements (TE). Can activate MYB25, MYB46, MYB58, MYB63, MYB83, MYB103, CESA4, LBD15, LBD30, ERF115, XCP1, XCP2, NAC010/SND3, KNAT7, ASL19 and ASL20 expression.
Gene References into Functions
  1. VND6 controls genes downstream that are involved in programmed cell death. PMID: 20952636
  2. A glucocorticoid-mediated posttranslational induction system of VND6 and VND7, is reported. PMID: 20488898
  3. VASCULAR-RELATED NAC-DOMAIN PROTEIN6 (VND6) and VND7, which are transcription factors required for TE differentiation, determine ASL19 and ASL20 expression in immature tracheary elements (TEs). PMID: 19088331
  4. Transcription switch for plant metaxylem and protoxylem vessel formation [Vnd6] [VASCULAR-RELATED NAC-DOMAIN6 ] PMID: 16103214
Subcellular Location
Nucleus.
Protein Families
Plant vascular related NAC-domain protein family
Tissue Specificity
Expressed in root inner metaxylem vessels and in hypocotyl vessels. Present in root developing xylems. Accumulates in the xylem but not in interfascicular fibers or pith cells in inflorescence stems. Absent from secondary xylem in roots.
Database Links

KEGG: ath:AT5G62380

STRING: 3702.AT5G62380.1

UniGene: At.55682

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