N Antibody

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Code	CSB-PA772943XA01RML
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Product Details

Full Product Name

Rabbit anti-Rice yellow stunt virus (RYSV) (Rice transitory yellowing virus) N Polyclonal antibody

Uniprot No.

Q86523

Target Names

Alternative Names

NNucleoprotein antibody; NP antibody; Nucleocapsid protein antibody; Protein N antibody

Raised in

Rabbit

Species Reactivity

Rice yellow stunt virus (RYSV) (Rice transitory yellowing virus)

Immunogen

Recombinant Rice yellow stunt virus (RYSV) (Rice transitory yellowing virus) N protein

Immunogen Species

Rice yellow stunt virus (RYSV) (Rice transitory yellowing virus)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Encapsidates the genome, protecting it from nucleases. If expressed without protein P it binds non-specifically RNA and therefore can bind it's own mRNA. Interaction with protein P abolishes any non-specific RNA binding, and prevents phosphorylation. The soluble N-P complex encapsidates specifically the genomic RNA, with protein N protecting the genome like a pearl necklace. The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as template for viral transcription and replication. Protein N binds protein P in the NC through a different interaction, and can be phosphorylated. Subsequent viral replication is dependent on intracellular concentration of newly synthesized protein N. During replication, encapsidation by protein N is coupled to RNA synthesis and all replicative products are resistant to nucleases.

Subcellular Location

Virion. Host cytoplasm.

Protein Families

Nucleorhabdovirus nucleocapsid protein family

Database Links

KEGG: vg:944310

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