ndc-80 Antibody

Code CSB-PA613757XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans ndc-80 Polyclonal antibody
Uniprot No.
Target Names
ndc-80
Alternative Names
ndc-80 antibody; W01B6.9Kinetochore protein ndc-80 antibody; CeNDC-80 antibody; Hec1 homolog antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans ndc-80 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Acts as a component of the essential kinetochore-associated ndc-80 complex, which is required for chromosome segregation in mitosis and meiosis and spindle checkpoint activity. Plays a role in kinetochore assembly and recruits the checkpoint protein mdf-2 and the spindly-like protein spdl-1 to unattached kinetochores. Mediates the formation of end-on kinetochore-microtubule attachments through recruitment of spdl-1. The ndc-80 complex synergistically enhances the affinity of the ska-1 complex for microtubules and may allow the ndc-80 complex to track depolymerizing microtubules.
Gene References into Functions
  1. Ndc80-tail dephosphorylation promotes stabilization of kinetochore-microtubule attachments via the Ska complex. PMID: 28535376
  2. Ndc80 complex bound to microtubules binds more tightly to beta-tubulin. PMID: 26941333
  3. KLP-7 acts through the Ndc80 complex to limit pole number in C. elegans oocyte meiotic spindle assembly. PMID: 26370499
  4. kinetochore dynein module inhibited microtubule binding by the Ndc80 complex; kinetochore dynein module directly regulated Ndc80, independently of phosphorylation by Aurora B kinase, and this regulation was required for accurate segregation PMID: 24231804
Subcellular Location
Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton.
Protein Families
NDC80/HEC1 family
Database Links

KEGG: cel:CELE_W01B6.9

STRING: 6239.W01B6.9.1

UniGene: Cel.12240

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