NDX Antibody

Code CSB-PA447934XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NDX Polyclonal antibody
Uniprot No.
Target Names
NDX
Alternative Names
NDX antibody; At4g03090 antibody; F4C21.1 antibody; T4I9.3 antibody; Nodulin homeobox antibody; NDX1 homeobox protein homolog antibody; AtNDX1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana NDX protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Regulates COOLAIR, a set of antisense transcripts originating from the 3' end of FLOWERING LOCUS C (FLC). Associates with single-stranded DNA that is part of an RNA-DNA hybrid, or R-loop, that covers the COOLAIR promoter. R-loop stabilization mediated by NDX inhibits COOLAIR transcription, which in turn modifies FLC expression.
Gene References into Functions
  1. identified a homeodomain protein, AtNDX (At4g03090), that regulates COOLAIR, a set of antisense transcripts originating from the 3' end of Arabidopsis FLOWERING LOCUS C (FLC); R-loop stabilization mediated by AtNDX inhibits COOLAIR transcription, which in turn modifies FLC expression PMID: 23641115
Subcellular Location
Nucleus. Nucleus, nucleolus.
Tissue Specificity
Expressed predominantly in dividing tissues such as young leaves, root tips, flower buds and embryos.
Database Links

KEGG: ath:AT4G03090

STRING: 3702.AT4G03090.1

UniGene: At.46863

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