NEDD1 Antibody

Code CSB-PA133108XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NEDD1 Polyclonal antibody
Uniprot No.
Target Names
NEDD1
Alternative Names
NEDD1 antibody; GCP-WD antibody; At5g05970 antibody; K18J17.16 antibody; Protein NEDD1 antibody; Neural precursor cell expressed developmentally down-regulated protein 1 homolog antibody; Protein GCP-WD antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana NEDD1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Regulates microtubules organization in a centrosome-independent manner. Required for the spindle to be positioned correctly and for the function of gamma-tubulin in organizing phragmoplast microtubules. Component of active gamma-tubulin ring complexes (gamma-TuRCs) associated with cortical microtubules in interphase cells. Mediates gamma-TuRC recruitment to the nucleation sites and is important for determining the ratio of branched to parallel nucleation. May mediate the localization of GCP2 and GCP3 to the nuclear envelope.
Gene References into Functions
  1. Knockdown of NEDD1 resulted in reduction of gamma-TuRC recruitment to cortical microtubules and total nucleation frequency, showing that GCP-WD/NEDD1 controls gamma-TuRC positioning and function PMID: 25438942
  2. NEDD1 homolog plays a critical role in MT organization during mitosis. PMID: 19383896
Subcellular Location
Nucleus envelope. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, phragmoplast. Cytoplasm, cytoskeleton, microtubule organizing center.
Tissue Specificity
Expressed in root meristematic cells.
Database Links

KEGG: ath:AT5G05970

STRING: 3702.AT5G05970.2

UniGene: At.32924

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