Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) Nep4 Polyclonal antibody

Uniprot No.

Q8T062

Target Names

Nep4

Alternative Names

Nep4 antibody; CG4058Neprilysin-4 antibody; EC 3.4.24.11 antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) Nep4 protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Metalloendoprotease which cleaves peptides at the amino side of hydrophobic residues - such as the hormones Akh and Dh31, and the neuropeptides Allatostatins (AST1, AST2, AST3 and AST4), Crz, Drosulfakinins (DSK-I and DSK-II), Lk, sNPF and the tachykinin peptides TK-1, TK-2, TK-4 and TK-5. Functions in female fertility, memory formation and may also act in regulating insulin signaling and food intake. Likely to be involved in controlling feeding behavior and the expression of insulin-like peptides by cleaving various regulatory peptides that include certain Drosulfakinins, Allatostatins and tachykinin peptides. Required in females for normal patterns of egg laying and hatching. Required in the dorsal paired medial neurons for the proper formation of long-term (LTM) and middle-term memories (MTM). Also required in the mushroom body neurons where it functions redundantly with neprilysins Nep2 and Nep3, in normal LTM formation.; Cleaves angiotensin-1 and tachykinin neuropeptide substance P. Functions in maintaining muscle integrity, possibly independently of its endopeptidase activity.

Gene References into Functions

Herein, the authors show that in Drosophila expression of insulin-like peptides is regulated by neprilysin 4 activity. PMID: 27919317
The NEP4 involved in middle-term memory (MTM) and long-term memory (LTM) and that their expression is required in the mushroom bodies and also in a single pair of closely connected neurons. PMID: 27629706
Overexpression of Nep4 leads to a severe muscle degeneration. PMID: 22583317
Results report the identification and first characterization of neprilysin 4 (NEP4) from Drosophila melanogaster. PMID: 19880729

Subcellular Location

[Isoform A]: Cell membrane; Single-pass type II membrane protein. Sarcoplasmic reticulum.; [Isoform B]: Cytoplasm.

Protein Families

Peptidase M13 family

Tissue Specificity

Expressed in the gonads and testes of adults, and the adult and larval brain (at protein level). In embryos, expressed in the pericardial, muscle founder and glia cells (at protein level). In stage 12 embryos, expressed in specific dorsal muscle founder c

Database Links

KEGG: dme:Dmel_CG4058

STRING: 7227.FBpp0083345

UniGene: Dm.1264

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Nep4 Antibody

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