NetB Antibody

Code CSB-PA638767XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) NetB Polyclonal antibody
Uniprot No.
Target Names
NetB
Alternative Names
NetB antibody; CG10521Netrin-B antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) NetB protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either fra or unc-5 receptors will lead to axon attraction or repulsion, respectively. While short-range repulsion requires both fra and unc-5 receptors, long-range repulsion only requires unc-5.
Gene References into Functions
  1. Data find that Abl interacts both physically and genetically with the Netrin receptor Frazzled, and that disrupting this interaction prevents Abl from promoting midline axon crossing. PMID: 23720041
  2. Netrin-B is specifically localized in photoreceptor axons owing to axonal release by lamina neurons L3 and capture by target neuron-associated Frazzled. PMID: 22794263
  3. Dscam1 functions to counter Drosophila sensory neuron dendritic targeting signals provided by secreted Netrin-B and Frazzled, a netrin receptor. Loss of Dscam1 function resulted in aberrant dendrite accumulation at a Netrin-B-expressing target. PMID: 21871804
  4. Dscam proteins function as attractive receptors for Netrin and also act in parallel to Frazzled/DCC. PMID: 18948420
Subcellular Location
Secreted, extracellular space, extracellular matrix. Cytoplasm, perinuclear region.
Tissue Specificity
At 24 hr after puparium formation (APF), detected in the most anterior (oldest) L3, L4 and L5 lamina neurons (at protein level). At 48 hr APF, expressed in all L3, L4 and L5 neurons with slightly higher expression in the L3 neurons (at protein level). At
Database Links

KEGG: dme:Dmel_CG10521

STRING: 7227.FBpp0291228

UniGene: Dm.3913

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