nfsB Antibody

Code CSB-PA339972XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) nfsB Polyclonal antibody
Uniprot No.
Target Names
nfsB
Alternative Names
nfsB antibody; dprA antibody; nfnB antibody; nfsI antibody; ntr antibody; b0578 antibody; JW0567 antibody; Oxygen-insensitive NAD(P)H nitroreductase antibody; EC 1.-.-.- antibody; Dihydropteridine reductase antibody; EC 1.5.1.34 antibody; FMN-dependent nitroreductase antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) nfsB protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred. Capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2,4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species.
Gene References into Functions
  1. investigation of flavin-independent Fenton reaction system; NfnB and Fpr were purified and showed catalytic activities of ferric reduction; characterization of these enzymes and their involvement in the Fenton reaction and ferric reduction are presented PMID: 20407804
  2. Data describe the interaction of Escherichia coli nitroreductase with the antibiotic nitrofurazone. PMID: 15684426
Protein Families
Nitroreductase family
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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