nfsB Antibody

Datasheet

Code	CSB-PA339972XA01ENV
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Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) nfsB Polyclonal antibody

Uniprot No.

P38489

Target Names

nfsB

Alternative Names

nfsB antibody; dprA antibody; nfnB antibody; nfsI antibody; ntr antibody; b0578 antibody; JW0567 antibody; Oxygen-insensitive NAD(P)H nitroreductase antibody; EC 1.-.-.- antibody; Dihydropteridine reductase antibody; EC 1.5.1.34 antibody; FMN-dependent nitroreductase antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) nfsB protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred. Capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2,4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species.

Gene References into Functions

investigation of flavin-independent Fenton reaction system; NfnB and Fpr were purified and showed catalytic activities of ferric reduction; characterization of these enzymes and their involvement in the Fenton reaction and ferric reduction are presented PMID: 20407804
Data describe the interaction of Escherichia coli nitroreductase with the antibiotic nitrofurazone. PMID: 15684426

Protein Families

Nitroreductase family

Database Links

KEGG: ecj:JW0567

STRING: 316385.ECDH10B_0644

Promotions

Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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nfsB Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background