ngb Antibody

Code CSB-PA015776XA01DIL
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Product Details

Full Product Name
Rabbit anti-Danio rerio (Zebrafish) (Brachydanio rerio) ngb Polyclonal antibody
Uniprot No.
Target Names
ngb
Alternative Names
ngb antibody; si:ch211-233n24.2 antibody; Neuroglobin antibody
Raised in
Rabbit
Species Reactivity
Danio rerio (Zebrafish) (Brachydanio rerio)
Immunogen
Recombinant Danio rerio (Zebrafish) (Brachydanio rerio) ngb protein
Immunogen Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Capable of penetrating cell membranes.
Gene References into Functions
  1. The zebrafish Neuroglobin can catalyze the reduction of nitrite to nitric oxide with a broad range of reaction rate constants. PMID: 26721561
  2. Data show that the neuroglobin (Ngb) protein was upregulated in the amacrine cells after optic nerve injury. PMID: 26427474
  3. Chimeric Myoglobin has a cell-membrane-penetrating activity similar to zebrafish Ngb. PMID: 21304818
  4. Neuroglobin appears to be associated with mitochondria-rich cell types and thus oxygen consumption rates, suggesting a myoglobin-like function of this protein in facilitated oxygen diffusion PMID: 15140880
  5. both zebrafish and chimeric ZHHH Ngb can penetrate cell membranes in the absence of Chariot PMID: 18416560

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Subcellular Location
Perikaryon. Cytoplasm.
Protein Families
Globin family
Tissue Specificity
Detected in brain, eye and gill, but not in muscle and blood (at protein level). Particularly high expression in the periventral zone of tectum opticum, with significant expression detected in white matter, preglomerular nucleus, posterior tubular nucleus
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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